PNU-176798

目录号: GC32378纯度: >98%

PNU-176798是一种抗菌剂，能够作用于大多数革兰氏阳性菌和厌氧菌的蛋白合成。

Cas No.: 428861-91-0

规格	价格	库存	数量
1mg	¥8,381.00	现货	1
5mg	¥13,405.00	现货	1
10mg	¥21,456.00	现货	1

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文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例

Nature
641, 529–536 (2025)
Nature
628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
Cell Research
33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

产品描述 Description

PNU-176798 is an antimicrobial agent, targeting protein synthesis in a wide spectrum of gram-positive and anaerobic bacteria.

PNU-176798 is an antimicrobial agent, with a minimum inhibitory concentration (MIC) of 1.4 μM for E. coli. PNU-176798 inhibits fMet-tRNA binding to the 70S ribosomes, with an IC50 of 32 μM. PNU-176798 also blocks translation, 70S initiation with IC50s of 0.53, and 32 μM, respectively. PNU-176798 inhibits peptidyl transferase (IC50, 40 μM), and the inhibition is more pronounced in the presence of elongation factor P (EF-P). PNU-176798 markedly inhibits the EF-G-mediated translocation of fMet-tRNA (IC50, 8 μM)[1].

[1]. Aoki H, et al. Oxazolidinone antibiotics target the P site on Escherichia coli ribosomes. Antimicrob Agents Chemother. 2002 Apr;46(4):1080-5.

实验参考方法 Experimental Reference Method

Kinase experiment:

Binding of the f[35S]Met-tRNA to E. coli 70S ribosomes is conducted and the initiation reaction mixtures are prepared without initiation factors and contain 6 mM magnesium acetate [Mg(Ac)2], 0.08 μM AUG, 30 mM NH4Cl, 10 mM Tris (pH 7.4), and 20 pmol of 70S ribosomes in a final volume of 60 μL. The reaction mixtures are incubated for 15 min at 35°C, and the reactions are terminated by addition of cold buffer A, washed with buffer A, and counted by liquid scintillation[1].

References:

[1]. Aoki H, et al. Oxazolidinone antibiotics target the P site on Escherichia coli ribosomes. Antimicrob Agents Chemother. 2002 Apr;46(4):1080-5.

产品文档 Product Documents

Purity:>98%

COA SDS Data Sheet

化学性质Chemical Properties

CAS 号

428861-91-0

SMILES

CC(NC[C@H]1CN(C2=CC=C(C3=NC=C(C#N)S3)C(F)=C2)C(O1)=O)=O

分子式

C₁₆H₁₃FN₄O₃S

分子量

360.36 g/mol

溶解性

Soluble in DMSO

保存条件

Store at -20°C

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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质量 (Mass)

体积 (Volume)

浓度 (Concentration)

分子量 (MW)

g/mol