Photocaged DAP can be genetically encoded as a protein in mammalian cells. Photocaged DAP can be used as an intermediate to capture biosynthetic acylase.
References:
[1]. Eiamthong B, et al. Discovery and Genetic Code Expansion of a Polyetlene Terephthalate (PET) drolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET. Angew Chem Int Ed Engl. 2022 Sep 12;61(37):e202203061.
[2]. Tang S, et al. Mechanism-based traps enable protease and drolase substrate discovery. Nature. 2022 Feb;602(7898):701-707.
















