磷酸酶抑制剂混合液III（Phosphatase Inhibitor Cocktail III (100× in DMSO)）有效抑制丝氨酸/苏氨酸和碱性磷酸酶。
Sample solution is provided at 25 µL, 10mM.
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Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: Cantharidin, (-)-p-Bromotetramisole oxalate, and Calyculin A form Discodermia calyx.
Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.