Peptide 74 is a synthetic peptide containing the prodomain sequence of matrix metalloproteinase (MMP). Peptide 74 inhibits the activated form of the 72-kDa type IV collagenase in vitro[1].
Peptide 74 (30 μM) reduces both A2058 and HT 1080 tumor cell invasion by 60-80%[1]. Peptide 74 (30 μM) shows no cytotoxic action and does not inhibit chemotaxis or affect HT1080 and A2058 human tumor cells number[1].
[1]. A Melchiori, et al. Inhibition of tumor cell invasion by a highly conserved peptide sequence from the matrix metalloproteinase enzyme prosegment. Cancer Res. 1992 Apr 15;52(8):2353-6.
[2]. P P Lee, et al. Functional role of matrix metalloproteinases (MMPs) in mammary epithelial cell development. J Cell Physiol. 2001 Jul;188(1):75-88.