PBFI AM, the cell membrane permeable fluorescent probe, acts as the K(+)-sensitive dye [1]. The interaction of buforin IIb with Jurkat cell membranes was studied by measuring intracellular K+ levels using PBFI AM [2]. PBFI AM can be used in flow cytometry analysis to investigate the correlation between cell apoptosis and potassium ion levels[3].
References:
[1] Kasner S E, Ganz M B. Regulation of intracellular potassium in mesangial cells: a fluorescence analysis using the dye, PBFI[J]. American Journal of Physiology-Renal Physiology, 1992, 262(3): F462-F467.
[2] Lee H S, Park C B, Kim J M, et al. Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide[J]. Cancer letters, 2008, 271(1): 47-55.
[3] Scoltock A B, Cidlowski J A. Activation of intrinsic and extrinsic pathways in apoptotic signaling during UV-C-induced death of Jurkat cells: the role of caspase inhibition[J]. Experimental cell research, 2004, 297(1): 212-223.
PBFI AM是一种细胞膜渗透性荧光探针，可作为钾离子敏感性染料使用[1]。通过使用PBFI AM检测细胞内钾离子浓度，可研究buforin IIb与Jurkat细胞膜的相互作用机制[2]。PBFI AM可应用于流式细胞分析，用于探究细胞凋亡与钾离子水平之间的关联[3]。