PAF C-18-d4

PAF C-18-d₄ contains four deuterium atoms at the 9, 9', 10, and 10' positions. It is intended for use as an internal standard for the quantification of PAF C-18 by GC- or LC-mass spectrometry. PAF C-18 is a naturally occurring phospholipid produced upon stimulation through two distinct pathways known as the "remodeling" and 'de novo' pathways.¹ It is less potent than PAF C-16 in the induction of platelet aggregation, but equipotent in activation of guinea pig macrophages.² PAF C-18 induces the release of PGE₂ and TXB₂ from albumin-elicited guinea pig macrophages, and enhances the spreading of plated macrophages.³ Pathological processes involving PAF include necrotizing enterocolitis,⁴ inflammation, asthma, and allergy.⁵

1.Prescott, S.M., Zimmerman, G.A., and McIntyre, T.M.Platelet-activating factorThe Journal of Biological Chemisty26517381-17384(1990) 2.Stewart, A.G., and Grigoriadis, G.Structure-activity relationships for platelet-activating factor (PAF) and analogs reveal differences between PAF receptors on platelets and macrophagesJournal of Lipid Mediators4299-308(1991) 3.Hartung, H.P.Acetyl glyceryl ether phosphorylcholine (platelet-activating factor) mediates heightened metabolic activity in macrophages. Studies on PGE, TXB2 and O2 production, spreading, and the influence of calmodulin-inhibitor W-7FEBS Letters160209-212(1983) 4.Wang, H., Tan, X.D., Qu, X.W., et al.Platelet-activating factor (PAF) up-regulates plasma and tissue PAF-acetylhydrolase activity in the rat: Effect of cycloheximidePediatric Research42597-603(1997) 5.Sturk, A., Wouter Ten Cate, J., Hosford, D., et al.The synthesis, catabolism, and pathophysiological role of platelet-activating factorAdvances in Lipid Research23219-276(1989)