Coumarin-caged nicotine. Releases nicotine when exposed to 390 ± 10 nm wavelength light (λmax = 404 nm). Suitable for two-photon uncaging at <900 nm (Maximum two-photon cross-section at 810 nm). Extinction coefficient (ε) 17,400 M-1cm-1. Evokes nicotinic currents in mouse brain slices under one and two-photon activation conditions.
Banala et al (2018) Photoactivatable drugs for nicotinic optopharmacology. Nat.Methods 15 347 PMID:29578537 |Yan et al (2018) Nicotinic cholinergic receptors in VTA glutamate neurons modulate excitatory transmission. Cell Rep. 23 2236 PMID:29791835 |Arvin et al (2019) Probing nicotinic acetylcholine receptor function in mouse brain slices via laser flash photolysis of photoactivatable nicotine. J.Vis.Exp. 143 e58873 PMID:30735191