Oregon Green™ 488 is a green fluorescent dye which is suitable for excitation with a 488nm light source [1]. Oregon Green™ 488 displays absorption/emission maximum of 490/514nm, respectively [2]. Oregon Green™ 488 has unchanging absorption and fluorescence properties in the physiological pH range, and it has been conjugated to antibodies and proteins for use as a fluorescent label [1].
References:
[1] Orte A, Crovetto L, Talavera E M, et al. Absorption and emission study of 2‘,7‘-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions [J]. The Journal of Physical Chemistry A, 2005, 109(5): 734-747.
[2] Sun W-C, Gee K R, Klaubert D H, et al. Synthesis of fluorinated fluoresceins [J]. The Journal of Organic Chemistry, 1997, 62(19): 6469-6475.
Oregon Green™ 488是一种绿色荧光染料，适用于488nm激光线进行激发[1]。Oregon Green™ 488的最大吸收/发射波长分别为490/514nm[2]。Oregon Green™ 488在生理pH范围内，可保持稳定的吸收和荧光特性，并且它可以与抗体和蛋白质偶联，用作荧光标记物[1]。