NFF-3 (trifluoroacetate salt)

NFF-3 is a fluorogenic substrate of matrix metalloproteinases (MMPs).^[1] NFF-3 is hydrolyzed rapidly by MMP-3 (k_cat/K_m = 218,000 s^-1*M^-1) and slowly by MMP-9 (k_cat/K_m = 10,100 s^-1*M^-1) with no significant hydrolysis by MMP-1 or MMP-2.^[1] NFF-3 can be used to differentiate MMP-3 activity from that of other MMPs.^1,2 

References:
[1].Giricz, O., Lauer, J.L., and Fields, G.B.Comparison of Metalloproteinase protein and activity profilingAnal. Biochem.409(1)37-45(2011).
[2].Nagase, H., Fields, C.G., and Fields, G.B.Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3)J. Biol. Chem.269(33)20952-20957(1994).