N6-Benzyladenosine-5'-O-diphosphate (sodium salt)

N⁶-Benzyladenosine-5'-O-diphosphate is a derivative of ADP and an active metabolite of the cytokinin N⁶-benzyladenine .^1,2,3 It inhibits the ATPase activities of mortalin, heat shock protein 70 (Hsp70), and heat shock cognate 71 kDa protein (Hsc70; apparent K_is = 86.51, 294.5, and 1,612 µM, respectively, for the human enzymes).² N⁶-Benzyladenosine-5'-O-diphosphate inhibits the proliferation of CEM, HL-60, and K562 leukemia, RPMI-8226 multiple myeloma, and MCF-7 breast cancer cells (IC⁵⁰s = 2, 1.3, 4, 4.5, and 8 µM, respectively).³ It has also been used in the synthesis of radiolabeled and unlabeled forms of N⁶-benzyladenosine 5'-(γ-thio)-triphosphate (N⁶-benzyl-ATPγS), which have been used to determine kinase activity.^4,5