N4-Methyl-dCTP (N4me-dCTP) is an analog of deoxycytidine 5'-triphosphate (dCTP) that is applied in DNA or RNA synthesis with PCR[1]. N4-Methyl-dCTP naturally exists in some bacteria[2]. N4-Methyl-dCTP (200µM) improves the output of DNA synthesis at GC-rich region [1], and N4-Methyl-dCTP shows better acceptance by polymerase in SNP detection[3] due to overcoming strong secondary structure that interferes with primer annealing[4].
References:
[1] Flores-Juárez, Cyntia R et al. “PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2'-deoxycytidine 5'-triphosphate.” *BioTechniques* vol. 61,4 175-182. 1 Oct. 2016, doi:10.2144/000114457
[2] Ehrlich, M et al. “DNA methylation in thermophilic bacteria: N4-methylcytosine, 5-methylcytosine, and N6-methyladenine.” *Nucleic acids research* vol. 13,4 (1985): 1399-412. doi:10.1093/nar/13.4.1399
[3] Yang, Zunyi et al. “Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems.” *Biology methods & protocols* vol. 5,1 bpaa004. 10 Feb. 2020, doi:10.1093/biomethods/bpaa004
[4]Swenson, Stephanie J, and Birgit Gemeinholzer. “Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing.” *PloS one* vol. 16,2 e0245611. 2 Feb. 2021, doi:10.1371/journal.pone.0245611
N4-Methyl-dCTP（N4me-dCTP）是脱氧胞苷5'-三磷酸（dCTP）的类似物，应用于DNA或RNA的PCR合成[1]。N4-Methyl-dCTP天然存在于某些细菌中。N4-Methyl-dCTP (200µM)提高了富GC区的DNA合成产量[1]，并且由于克服了干扰引物退火的强二级结构[4]，N4-Methyl-dCTP在SNP检测中被聚合酶更好地接受[3]。