N1-Me-pUTP,100mM Sodium Solution is a methyl modified form of naturally occurring pseudouridine (pUTP), which is commonly used in RNA therapy and RNA vaccine synthesis[1, 2]. N1-Me-pUTP can be used in conjunction with lipid nanoparticle (LNP) encapsulation to optimize the stability and efficacy of COVID-19 vaccines[3]. N1-Me-pUTP-modified mRNA can lead to reduced immune response and increased protein expression in vivo[4, 5].
References:
[1] Parr C J C, Wada S, Kotake K, et al. N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells[J]. Nucleic Acids Research, 2020, 48(6): e35-e35.
[2] Andries O, Mc Cafferty S, De Smedt S C, et al. N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice[J]. Journal of Controlled Release, 2015, 217: 337-344.
[3] Luo Z, Lin Y, Meng Y, et al. Spleen-targeted mRNA vaccine doped with manganese adjuvant for robust anticancer immunity in vivo[J]. ACS nano, 2024, 18(44): 30701-30715.
[4] Chen T H, Potapov V, Dai N, et al. N 1-methyl-pseudouridine is incorporated with higher fidelity than pseudouridine in synthetic RNAs[J]. Scientific Reports, 2022, 12(1): 13017.
[5] Mokuda S, Watanabe H, Kohno H, et al. N1-methylpseudouridine-incorporated mRNA enhances exogenous protein expression and suppresses immunogenicity in primary human fibroblast-like synoviocytes[J]. Cytotechnology, 2022, 74(4): 503-514.
N1-Me-pUTP,100mM Sodium Solution是天然存在的假尿苷（pUTP）的甲基修饰物，常用于RNA治疗和RNA疫苗合成[1, 2]。N1-Me-pUTP能够与脂质纳米颗粒（LNP）包封联用，优化COVID-19疫苗的稳定性和效力[3]。N1-Me-pUTP修饰的mRNA能够导致体内免疫反应降低和蛋白质表达增加[4, 5]。