N-Succinimidyl-S-acetylthioacetate is a protein-modifying agent.1 It introduces sulfhydryl groups to proteins via S-acetylthioacetylation of free amine groups, which can be subsequently deprotected by deacetylation to reveal an unprotected thiol. N-Succinimidyl-S-acetylthioacetate has been used in conjunction with the protein cross-linking reagent SMCC in the conjugation of proteins with IgG antibodies for use in ELISAs.1,2
1.Duncan, R.J., Weston, P.D., and Wrigglesworth, R.A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassayAnal. Biochem.32(1)68-73(1983) 2.Nanda, S., Muralidhar, K., and Kar, S.K.Thermostable α-amylase conjugated antibodies as probes for immunodetection in ELISAJ. Immunoassay Immunochem.23(3)327-345(2002)