Nε-(1-Carboxymethyl)-L-lysine是一种独特的蛋白质翻译后修饰（PTM），由赖氨酸残基的非酶糖基化反应产生。
Cas No.:5746-04-3
Sample solution is provided at 25 µL, 10mM.
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Nε-(1-Carboxymethyl)-L-lysine is a unique post-translational modification (PTM) of proteins that is generated by the non-enzymatic glycation of lysine residues [2]. The levels of CML in peripheral blood and circulating extracellular vesicles (EVs) can distinguish between early and middle-stage Alzheimer's disease[2]. Nε-(1-Carboxymethyl)-L-lysine has been employed as a pertinent marker that reflects the occurrence and level of process-induced AGEs in food or of those endogenously formed[3].
In vitro, Nε-(1-Carboxymethyl)-L-lysine treatment at 50μM for 24 hours enhanced the expression of cancer stem cell markers in JJ012 cells, leading to the activation of the RAGE/NF-κB signaling pathway and the inhibition of the AKT signaling pathway[4]. Treatment with 10μM Nε-(1-Carboxymethyl)-L-lysine and 15mM D-glucose for 6 days significantly increased the mRNA expression of G6Pase in LO2 liver cells, decreased the phosphorylation level of AMPK, and promoted cellular lipid biosynthesis and fatty acid β-oxidation[5]. Treatment of U2OS cells with 50μM Nε-(1-Carboxymethyl)-L-lysine for 24 hours induced cell migration and invasion, and stimulated the expression of RAGE, ERK and NFκB[6].
References:
[1] Fu M X, Requena J R, Jenkins A J, et al. The advanced glycation end product, N∊-(Carboxymethyl) lysine, is a product of both lipid peroxidation and glycoxidation reactions (∗)[J]. Journal of Biological Chemistry, 1996, 271(17): 9982-9986.
[2] Haddad M, Perrotte M, Landri S, et al. Circulating and extracellular vesicles levels of N-(1-Carboxymethyl)-L-Lysine (CML) differentiate early to moderate Alzheimer’s disease[J]. Journal of Alzheimer’s Disease, 2019, 69(3): 751-762.
[3] Tareke E, Forslund A, Lindh C H, et al. Isotope dilution ESI-LC-MS/MS for quantification of free and total Nε-(1-Carboxymethyl)-l-Lysine and free Nε-(1-Carboxyethyl)-l-Lysine: Comparison of total Nε-(1-Carboxymethyl)-l-Lysine levels measured with new method to ELISA assay in gruel samples[J]. Food Chemistry, 2013, 141(4): 4253-4259.
[4] Chang T Y, Lan K C, Wu C H, et al. Nε-(1-Carboxymethyl)-L-lysine, an advanced glycation end product, exerts malignancy on chondrosarcoma via the activation of cancer stemness[J]. Archives of Toxicology, 2023, 97(8): 2231-2244.
[5] Wu Y, Wu Y, Zhang Z, et al. N ε-(Carboxymethyl) lysine (CML) Promotes Disorders of Glucose and Lipid Metabolism in Human Hepatic LO2 Cells[J]. ACS Food Science & Technology, 2023, 3(10): 1635-1645.
[6] Chang T Y, Lan K C, Wu C H, et al. Nε-(1-carboxymethyl)-L-lysine/RAGE signaling drives metastasis and cancer stemness through ERK/NFκB axis in osteosarcoma[J]. International Journal of Biological Sciences, 2024, 20(3): 880.
Nε-(1-Carboxymethyl)-L-lysine是一种独特的蛋白质翻译后修饰（PTM），由赖氨酸残基的非酶糖基化反应产生[1]。外周血和循环细胞外囊泡（EVs）中Nε-(1-Carboxymethyl)-L-lysine的水平能够区分早期和中期阿尔茨海默病[2]。Nε-(1-Carboxymethyl)-L-lysine已被用作一个相关标志物，用于反映食品中加工诱导的AGEs或内源性形成的AGEs的发生和水平[2]。
在体外，使用50μM的Nε-(1-Carboxymethyl)-L-lysine处理JJ012细胞24小时，增强了癌症干细胞标志物的表达，导致RAGE/NF-κB信号通路激活以及AKT信号通路抑制[4]。使用10μM的Nε-(1-Carboxymethyl)-L-lysine和15mM的D-葡萄糖处理LO2肝细胞6天，显著增加了G6Pase的mRNA表达，降低了AMPK的磷酸化水平，并促进了细胞脂质生物合成和脂肪酸β-氧化[5]。使用50μM的Nε-(1-Carboxymethyl)-L-lysine处理U2OS细胞24小时，诱导了细胞迁移和侵袭，并刺激了RAGE、ERK和NFκB的表达[6]。
| Cell experiment [1]: | |
Cell lines | JJ012 cells |
Preparation Method | JJ012 cells were maintained in DMEM/α-MEM (50%/50%) medium containing 10% fetal bovine serum (FBS) in a humidified 5% CO2 incubator (95% air/5% CO2) at 37°C. JJ012 cells were seeded at 4×105 cells per well in six-well plates for 24h, and were treated 50μM Nε-(1-Carboxymethyl)-L-lysine for another 24h. The protein expression levels of cancer stem cell markers, including MRP1, MDR1, CD44, ALDH1A1, and NANOG in both JJ012 cells with or without Nε-(1-Carboxymethyl)-L-lysine (50μM) treatment were analyzed by Western blotting. |
Reaction Conditions | 50μM; 24h |
Applications | Nε-(1-Carboxymethyl)-L-lysine treatment enhanced cancer stem cell markers expression in JJ012 cells. |
References: | |
| Cas No. | 5746-04-3 | SDF | |
| 别名 | NΕ-(1-羧甲基)-L-赖氨酸 | ||
| Canonical SMILES | OC(CNCCCC[C@H](N)C(O)=O)=O | ||
| 分子式 | C8H16N2O4 | 分子量 | 204.2 |
| 溶解度 | PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8972 mL | 24.4858 mL | 48.9716 mL |
| 5 mM | 979.4 μL | 4.8972 mL | 9.7943 mL |
| 10 mM | 489.7 μL | 2.4486 mL | 4.8972 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >95.00% Appearance: A solid
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