Myristic Acid Alkyne is a click chemistry reagent with a terminal alkyne group that can undergo Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions[1]. Myristic Acid Alkyne can be used to identify and characterize post-translationally myristoylated proteins[2]. Myristic Acid Alkyne is a long hydrocarbon chain (C14) with an ω-terminal alkyne, and it is a synthetic derivative of myristic acid[3].
Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecule can be easily labelled with small molecules, such as fluorescent dyes, biotin, etc using click chemistry method.
Click chemistry reaction takes place between two components: azide and alkyne (terminal acetylene). Both azido and alkyne groups are nearly never encountered in natural biomolecules. Hence, the reaction is highly bioorthogonal and specific.
References:
[1] Agrahari A K, Bose P, Jaiswal M K, et al. Cu (I)-catalyzed click chemistry in glycoscience and their diverse applications[J]. Chemical Reviews, 2021, 121(13): 7638-7956.
[2] Xu Y, Lin H. Use of alkyne-tagged myristic acid to detect N-terminal myristoylation[M]Methods in Enzymology. Academic Press, 2023, 684: 191-208.
[3] Hammerer L, Winkler C K, Kroutil W. Regioselective biocatalytic hydroxylation of fatty acids by cytochrome P450s[J]. Catalysis Letters, 2018, 148: 787-812.
肉豆蔻酸炔（Myristic Acid Alkyne）是一种点击化学试剂，末端炔基可以发生Cu(I)催化的叠氮化物-炔环加成反应（CuAAC）[1]。Myristic Acid Alkyne可用识别和表征翻译后肉豆蔻酰化蛋白质[2]。Myristic Acid Alkyne是带有ω末端炔基的长烃链（C14），是肉豆蔻酸的合成衍生物[3]。
点击化学是一种多功能反应，可用于合成各种缀合物。事实上，任何生物分子都可以使用点击化学方法轻松地用小分子标记，例如荧光染料、生物素等。
点击化学反应发生在两种组分之间：叠氮化物和炔烃（末端乙炔）。叠氮基和炔基在天然生物分子中几乎从未遇到过。因此，该反应具有高度生物正交性和特异性。