Metronidazole-d4 is intended for use as an internal standard for the quantification of metronidazole by GC- or LC-MS. Metronidazole is an antibiotic that has activity against anaerobic bacteria and protozoa including T. vaginalis, E. histolytica, G. lamblia, C. difficile, and H. pylori.1 It reduces the growth of E. coli in vitro (MIC = 128 mg/L under anaerobic conditions).2 In vivo, metronidazole reduces viable counts of B. fragilis in a rabbit model of infection. Formulations containing metronidazole have been used in the treatment of various infections including H. pylori and C. difficile.
1.Samuelson, J.Why metronidazole is active against both bacteria and parasitesAntimicrob. Agents Chemother.43(7)1533-1541(1999) 2.Rylander, M., Holm, S.E., Brorson, J.-E., et al.Activity of metronidazole on Bacteroides fragilis and/or Escherichia coli in vitro and in vivoJ. Antimicrob. Chemother.7(3)257-267(1981)