Malantide is a synthetic peptide derived from the β-subunit of human phosphorylase kinase and is a fluorescent substrate for PKA and PKC.[1],[2] Upon phosphorylation by PKA or PKC, the intrinsic tyrosine fluorescence decreases and can be used to quantify PKA and PKC activity. Malantide displays an emission at 302 nm upon excitation at 285 nm.
References:
[1].Malencik, D.A., and Anderson, S.R.Characterization of a fluorescent substrate for the adenosine 3',5'-cyclic monophosphate-dependent protein kinaseAnal. Biochem.132(1)34-40(1983).
[2].Zhao, Z.H., Malencik, D.A., and Anderson, S.R.Characterization of a new substrate for protein kinase C: Assay by continuous fluorometric monitoring and high performance liquid chromatographyBiochem. Biophys. Res. Commun.176(3)1454-1461(1991).