m6A-ATP,100mM Sodium Solution is the N6-modified ATP derivative, acting as the adenosine agonist with an ED50 value of 17250nM[1]. m6A-ATP,100mM Sodium Solution can be used to synthesize oligopeptide chains in vitro and serves as a crucial substrate for in vitro oligopeptide synthesis. Both unmethylated and N6-methylated RNA sequences were generated through in vitro transcription, utilizing ATP and m6A-ATP as distinct nucleotide precursors, respectively[2]. m6A-ATP,100mM Sodium Solution can be used as raw materials to synthesize methylated mRNA and non-coding RNAs (ncRNAs), and m6A-modified RNA has increased stability and participates in translation and intracellular localization regulation[3]. m6A-ATP,100mM Sodium Solution can affect the ATP probe labeling efficiency of GSK3α. The ATP probe labeling efficiencies for GSK3α were reduced by 15%, 44%, and 64% at concentrations of 10, 100, and 200μM m6A-ATP, respectively, with concentration-dependent inhibition[4]. Incubation with 250μM m6A-ATP at physiological temperature (37°C) for 2 hours facilitated GST-tagged GSK3β-mediated phosphorylation of a glycogen synthase 1-derived peptide substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE)[4].
References:
[1] Ribeiro J A, Sebastião A M. On the type of receptor involved in the inhibitory action of adenosine at the neuromuscular junction[J]. British journal of pharmacology, 1985, 84(4): 911.
[2] Xiao Y L, Liu S, Ge R, et al. Transcriptome-wide profiling and quantification of N 6-methyladenosine by enzyme-assisted adenosine deamination[J]. Nature biotechnology, 2023, 41(7): 993-1003.
[3]Sendinc E, Shi Y. RNA m6A methylation across the transcriptome[J]. Molecular cell, 2023, 83(3): 428-441.
[4] Dong X, Sun J, Miao W, et al. Proteome-Wide Characterizations of N 6-Methyl-Adenosine Triphosphate-and N 6-Furfuryl-Adenosine Triphosphate-Binding Capabilities of Kinases[J]. Analytical chemistry, 2021, 93(39): 13251-13259.
m6A-ATP,100mM Sodium Solution是一种N6修饰的ATP衍生物，作为腺苷激动剂，ED50值为17250nM[1]。m6A-ATP,100mM Sodium Solution可作为体外寡肽合成的重要底物，用于体外合成寡肽链。通过体外转录，分别以ATP和m6A-ATP作为不同的核苷酸前体，可生成未甲基化和N6甲基化的RNA序列[2]。m6A-ATP,100mM Sodium Solution可作为合成甲基化mRNA和非编码RNA（ncRNAs）的原料，m6A修饰的RNA稳定性增强，参与翻译过程和细胞内定位调控[3]。m6A-ATP,100mM Sodium Solution能够影响GSK3α的ATP探针标记效率，在10、100和200μM浓度的m6A-ATP处理后，GSK3α的ATP探针标记效率分别降低了15%、44%和64%，表现出浓度依赖性抑制作用[4]。当使用250μM m6A-ATP在生理温度（37°C）下孵育2小时，可促进GST标记-GSK3β介导的糖原合成酶1衍生肽底物（YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE）的磷酸化反应[4]。