Luteolinidin chloride是一种具有抗氧化活性的脱氧花青素，可以从Sorghum bicolor植物中分离得到。
Cas No.:1154-78-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Luteolinidin chloride is an antioxidative deoxyanthocyanidin that can be isolated from the plant Sorghum bicolor[1-2]. Luteolinidin chloride is an effective CD38 inhibitor (Ki=11.4µM) and protects the heart from ischemia/reperfusion injury by preserving endothelial nitric oxide synthase function and preventing endothelial dysfunction [3-4].
In vitro, MC-65 neuronal cells were treated with Luteolinidin chloride (10–20µM) for 72 hours, followed by the induction of Aβ42 expression under tetracycline-free conditions to generate toxicity. Luteolinidin chloride protected MC-65 cells from Aβ42-induced damage, significantly increased cell survival rates, and enhanced mitochondrial function[5]. Isolated perfused rat hearts were pretreated with Luteolinidin chloride (5–50µM) for 20 minutes, followed by 30 minutes of global ischemia and 30-120 minutes of reperfusion. Luteolinidin chloride significantly improved left ventricular systolic function and coronary flow, while reducing myocardial infarction area[6].
References:
[1] Boslett J J. CD38 in the Heart: Effects of CD38 Activation on Post-Ischemic Injury[D]. The Ohio State University, 2017.
[2] Webb MR, Min K, Ebeler SE. Anthocyanin Interactions with DNA: Intercalation, Topoisomerase I Inhibition and Oxidative Reactions. J Food Biochem. 2008 Sep 23;32(5):576-596.
[3] Bianco-Gomes AC, Nogueira LDS, Bono-Lopes NVM, et al. Dry heat and pressure favor bioactive compounds preservation and peptides formation in sorghum [Sorghum bicolor (L.) Moench]. Curr Res Food Sci. 2022 Jan 4;5:117-124.
[4] Yang SY, Kim JH, Su X, et al. The Luteolinidin and Petunidin 3-O-Glucoside: A Competitive Inhibitor of Tyrosinase. Molecules. 2022 Sep 4;27(17):5703.
[5] Abdulraheem RA, Danazumi AU, Nitschke P, et al. 3-Deoxyanthocyanidins inhibit β-amyloid aggregation, toxicity, and mitochondrial dysfunction: Evidence from MC-65 cells and molecular dynamics simulations. Free Radic Biol Med. 2026 Feb 1;247:213-223.
[6] Boslett J, Hemann C, Zhao YJ, et al. Luteolinidin Protects the Postischemic Heart through CD38 Inhibition with Preservation of NAD(P)(H). J Pharmacol Exp Ther. 2017 Apr;361(1):99-108.
Luteolinidin chloride是一种具有抗氧化活性的脱氧花青素，可以从Sorghum bicolor植物中分离得到[1-2]。Luteolinidin chloride是一种有效的CD38（Ki=11.4μM）抑制剂，通过保护内皮型一氧化氮合酶功能和预防内皮功能障碍来保护心脏免受缺血/再灌注损伤[3-4]。
在体外，Luteolinidin chloride（10–20μM）处理MC-65神经细胞72小时，随后在无四环素条件下诱导Aβ42表达以产生毒性，Luteolinidin chloride保护MC-65细胞免受Aβ42造成的损伤，显著提高细胞存活率，同时增强线粒体功能[5]。Luteolinidin chloride （5–50μM）预处理离体灌注大鼠心脏20分钟，随后进行30分钟全局性缺血及30-120分钟再灌注，显著改善左心室收缩功能与冠脉流量，同时减少心肌梗死面积[6]。
| Cell experiment [1]: | |
Cell lines | MC-65 neuronal cells (a human neuroblastoma cell line conditionally expressing the amyloid precursor protein C-terminal fragment) |
Preparation Method | MC-65 cells were cultured in DMEM/F-12 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin/streptomycin, 5μg/mL tetracycline, and 4mg/mL G418 at 37°C under 5% CO₂. To induce Aβ42-associated toxicity, cells were incubated in Opti-MEM medium without tetracycline. Cells were treated with Luteolinidin chloride (10–20μM) under the No-Tet condition. |
Reaction Conditions | 10-20μM; 72h. |
Applications | Luteolinidin chloride (10-20μM) significantly rescued MC-65 cells from Aβ42-induced toxicity. Luteolinidin chloride also ameliorated mitochondrial dysfunction, increasing ATP levels. |
References: | |
| Cas No. | 1154-78-5 | SDF | |
| 别名 | 木犀草定氯化物 | ||
| Canonical SMILES | OC1=CC2=[O+]C(C3=CC=C(O)C(O)=C3)=CC=C2C(O)=C1.[Cl-] | ||
| 分子式 | C15H11ClO5 | 分子量 | 306.7 |
| 溶解度 | DMSO : 50 mg/mL (163.03 mM; Need ultrasonic) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2605 mL | 16.3026 mL | 32.6052 mL |
| 5 mM | 652.1 μL | 3.2605 mL | 6.521 mL |
| 10 mM | 326.1 μL | 1.6303 mL | 3.2605 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet