Lupiwighteone is an isoflavone present widely in wild-growing plants, with antioxidant, antimicrobial and anticancer effects. Lupiwighteone induces caspase-dependent and -independent Apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway[1][2].
Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC50s of 23.7 μM and 21 μM, respectively[2].
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells[2].
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis[2].
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells[2].
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production[2].
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells[2].
Cell Viability Assay[2]
| Cell Line: | DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero |
| Concentration: | 2 μM, 5 μM, 25 μM, 50 μM, 100 μM |
| Incubation Time: | 72 hours |
| Result: | Had inhibition on the survival of many cancer cell lines and HUVEC. |
Cell Cycle Analysis[2]
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Induced cell cycle arrest. |
Apoptosis Analysis[2]
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Induced apoptosis. |
Western Blot Analysis[2]
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner. |
[1]. Won YS, et al. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway. Food Chem Toxicol. 2020 Jan;135:110863.
[2]. Ren J, et al. Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells. Anticancer Drugs. 2015 Jul;26(6):599-611.
[3]. Li XL, Sui L, Lin FH, Lian Y, Ai LZ, Zhang Y. Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice. Pharm Biol. 2019;57(1):226-230.