Loliolide是一种具有多种生物活性的单萜内酯。
Cas No.:5989-02-6
Sample solution is provided at 25 µL, 10mM.
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Loliolide is a monoterpene lactone with diverse biological activities. Loliolide is widely found in marine algae, terrestrial organisms, fungi, and certain animal organisms, and has a wide range of biological activities, including anti-inflammatory, antioxidant, anticancer, neuroprotective, antidiabetic, antidepressant, immunosuppressive, antiviral, and allelopathic effects[1].
In vitro, Loliolide did not induce cytotoxicity in HaCaT keratinocytes at concentrations of 0-62.5µg/mL, and in IFN-γ/TNF-α-stimulated HaCaT cells, it dose-dependently restored cell viability, reduced intracellular ROS, downregulated cytokines and chemokines, and inhibited NF-κB signaling by suppressing p-IκBα/p65 expression and p65 nuclear translocation[2]. Loliolide showed no cytotoxicity in SH-SY5Y cells at 1–100µM and exerted neuroprotective effects against 100µM 6-OHDA-induced toxicity, increasing cell viability by 23.70±7.77% and 41.06±6.31% at 50 and 100µM, respectively[3].
In vivo, Loliolide at 6.25, 12.5, and 25µg/mL for 1h dose-dependently inhibited UVB-induced ROS, NO production, lipid peroxidation, and cell death in zebrafish[4].
References:
[1] Farzeen I, Zafar S, Zafar N, Fatima S, Nazir MM, Ashraf A. Loliolide: green monoterpenoid lactone with therapeutic and ecological significance-a comprehensive review with computational validation. Mol Divers.
[2] Han EJ, Fernando IPS, Kim HS, et al. (-)-Loliolide Isolated from Sargassum horneri Suppressed Oxidative Stress and Inflammation by Activating Nrf2/HO-1 Signaling in IFN-γ/TNF-α-Stimulated HaCaT Keratinocytes. Antioxidants (Basel). 2021;10(6):856.
[3] Silva J, Alves C, Martins A, et al. Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum. Int J Mol Sci. 2021;22(4):1888.
[4] Wang L, Kim HS, Je JG, et al. In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, Sargassum horneri. Molecules. 2021;26(22):6898.
Loliolide是一种具有多种生物活性的单萜内酯。Loliolide广泛存在于海洋藻类、陆生生物、真菌和某些动物中，并具有广泛的生物活性，包括抗炎、抗氧化、抗癌、神经保护、抗糖尿病、抗抑郁、免疫抑制、抗病毒和化感作用[1]。
体外实验中，在0-62.5µg/mL浓度范围内，Loliolide对HaCaT角质形成细胞无细胞毒性；在IFN-γ/TNF-α刺激的HaCaT细胞中，其可剂量依赖性恢复细胞活力、降低细胞内ROS水平、下调细胞因子和趋化因子表达，并通过抑制p-IκBα/p65表达及p65的核转位而抑制NF-κB信号通路[2]。Loliolide在1-100µM范围内对SH-SY5Y细胞无细胞毒性，并对100µM 6-OHDA诱导的毒性具有神经保护作用，在50µM和100µM时分别使细胞活力提高23.70±7.77%和41.06±6.31%[3]。
体内实验中，Loliolide在6.25、12.5和25µg/mL浓度下处理1小时，可剂量依赖性抑制UVB诱导的斑马鱼体内ROS和NO的产生，以及脂质过氧化和细胞死亡[4]。
| Cell experiment [1]: | |
Cell lines | HaCaT (human epidermal keratinocytes) |
Preparation Method | Cells were seeded in 96-well plates and incubated for stabilization. Cells were then treated with different concentrations of Loliolide and after 1h, cells were stimulated with IFN-γ/TNF-α for 24h. |
Reaction Conditions | 0-62.5ug/ml |
Applications | Loliolide did not induce cytotoxicity in HaCaT keratinocytes at any of the tested concentrations. In IFN-γ/TNF-α-stimulated HaCaT keratinocytes, Loliolide dose-dependently restored cell viability, reduced intracellular ROS, downregulated cytokines and chemokines, and inhibited NF-κB signaling by suppressing p-IκBα/p65 expression and p65 nuclear translocation. |
| Animal experiment [2]: | |
Animal models | Zebrafish |
Preparation Method | The zebrafish larvae were treated with Loliolide (6.25, 12.5, and 25μg/mL) for 1h. The UVB-irradiated zebrafish larvae were further incubated for 6h. The ROS levels, cell death, NO production, and lipid peroxidation of UVB-irradiated zebrafish were determined. |
Dosage form | 6.25, 12.5, and 25μg/mL; 1h |
Applications | UVB significantly increased the ROS levels of zebrafish, and Loliolide effectively and dose-dependently reduced ROS levels of zebrafish. Loliolide significantly suppressed cell death in UVB-irradiated zebrafish in a dose-dependent manner. The NO generation of UVB-irradiated zebrafish was increased to 295.95% compared to non-irradiated zebrafish (100%), loliode decreased the NO levels to 276.63, 246.29, and 155.14% at the doses of 6.25, 12.5, and 25μg/mL, respectively. Loliolide suppressed lipid peroxidation stimulated by UVB irradiation in zebrafish in a dose-dependent manner. |
References: | |
| Cas No. | 5989-02-6 | SDF | |
| 别名 | 地芰普内酯 | ||
| Canonical SMILES | C[C@]12C(C(C)(C[C@@H](C1)O)C)=CC(O2)=O | ||
| 分子式 | C11H16O3 | 分子量 | 196.2 |
| 溶解度 | Methanol : 58.87 mg/mL (299.99 mM; Need ultrasonic and warming) | 储存条件 | Store at -20°C,protect from light and air |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL |
| 10 mM | 509.7 μL | 2.5484 mL | 5.0968 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet