Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare stock solution of Sorensen's buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4 (9.1 g/L).
2. Cover the smear with Leishman stain for 2 min.
3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min.
4. Wash off with running water and dry the smear (5 min).
[1]. S Gajendra, et al. Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int J Lab Hematol. 2015 Dec;37(6):774-82.