Lactoyl Phenylalanine is a secondary metabolite formed by the action of cytoplasmic non-specific dipeptidase (CNDP 2) between lactic acid and phenylalanine [1]. Lactoyl Phenylalanine can reduce obesity and improve glucose tolerance by inhibiting appetite and maintaining energy balance [2].
In vivo, Lactoyl Phenylalanine (50mg/kg/d; i.p.; 30 days) specifically inhibits energy intake in obese mice without affecting energy expenditure pathways, and improves blood sugar balance, while these effects are not observed in lean mice [2]. Lactoyl Phenylalanine (10, 20, 30mg/kg/day; intravenous) treatment for 7 days can significantly improve the motor function of mice and significantly reduce fat accumulation at the injured site [3].
References:
[1] Hinton Jr A, Neikirk K, Le H, et al. N-lactoyl phenylalanine suppresses appetite and obesity with important implications for aging and age-related diseases[J]. Aging advances, 2024, 1(2): 172-173.
[2] Li VL, He Y, Contrepois K, Liu H, Kim JT, Wiggenhorn AL, Tanzo JT, Tung AS, Lyu X, Zushin PH, Jansen RS, Michael B, Loh KY, Yang AC, Carl CS, Voldstedlund CT, Wei W, Terrell SM, Moeller BC, Arthur RM, Wallis GA, van de Wetering K, Stahl A, Kiens B, Richter EA, Banik SM, Snyder MP, Xu Y, Long JZ. An exercise-inducible metabolite that suppresses feeding and obesity. Nature. 2022 Jun;606(7915):785-790.
[3] Ying W, Weng W, Wang P, Pan C, Qiu J, Huang Q, Zhan G, Chen X. N-Lactoyl-Phenylalanine modulates lipid metabolism in microglia/macrophage via the AMPK-PGC1α-PPARγ pathway to promote recovery in mice with spinal cord injury. J Neuroinflammation. 2025 Jun 27;22(1):167.
Lactoyl Phenylalanine是一种由乳酸和苯丙氨酸通过胞质非特异性二肽酶（CNDP 2）形成的次级代谢产物 [1]。Lactoyl Phenylalanine能够通过抑制食欲和维持能量平衡的作用来减少肥胖并改善葡萄糖耐量 [2]。
在体内，Lactoyl Phenylalanine (50mg/kg/d, i.p.) 治疗肥胖小鼠30天，能够特异性地抑制能量摄入而不影响能量消耗途径，并且改善血糖平衡，而这些效果在瘦小鼠中并未表现出来 [2]。Lactoyl Phenylalanine（10, 20, 30mg/kg/day; i.v.）治疗7天能够显著改善小鼠的运动功能，并显著减少受伤部位的脂肪堆积 [3] 。