L-Homopropargyl glycine is a reactive methionine analog that contains an alkyne moiety. It is readily inserted into newly-synthesized proteins in place of methionine.1,2,3 L-Homopropargyl glycine can then be labeled or captured through click chemistry.2,4 This approach represents a fast, sensitive, and non-radioactive alternative to [35S]-methionine for the detection of nascent protein synthesis.
1.Beatty, K.E., and Tirrell, D.A.Two-color labeling of temporally defined protein populations in mammalian cellsBioorg. Med. Chem. Lett.18(22)5995-5999(2008) 2.Ngo, J.T., and Tirrell, D.A.Non-canonical amino acids in the interrogation of cellular protein synthesisAcc. Chem. Res.44(9)677-685(2011) 3.Landgraf, P., Antileo, E.R., Schuman, E.M., et al.BONCAT: Metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomesSite-specific protein labeling1266199-215(2015) 4.Dieterich, D.C., Hodas, J.J.L., Gouzer, G., et al.In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neuronsNat. Neurosci.13(7)897-905(2010)