Isovitexin extracted from rice hulls of Oryza sativa, possesses various biological activities, including anti-inflammatory and antioxidant activities[1].
In vitro，Isovitexin (5-20μM; 24h) protected HaCaT cells from H2O2-induced oxidative damage, alleviated H2O2-induced apoptosis[2]. Pretreatment HaCaT cells with 10-50μM Isovitexin for 30min completely abolished PM2.5-induced elevation of ROS levels[3].
In vivo, Isovitexin (50 or 100mg/kg; i.p.; once) pretreatment decreased TNF-α and IL-6 production, ROS generation, and MPO and MDA content, while increased SOD and GSH levels and inhibited the protein expression of iNOS and COX-2 in LPS-induced acute lung injury (ALI) mice[4]. Isovitexin (5mg/kg; oral) restored bone mass and architecture of trabecular bones (femur metaphysis and fifth lumbar vertebra/L5) and cortical bones (femur diaphysis) in ovariectomized (OVX) osteopenic mice. Isovitexin has an oral bioavailability of 14.58%[5].
References:
[1] Kang, Jie, et al. "Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities." Food chemistry 128.1 (2011): 152-157.
[2] Lv, Xuechun, et al. "The anti-apoptosis effect of isovitexin on human keratinocytes by regulating miR-98-5p/Bcl-2/Bcl-xL and MAPKs/NF-κB signaling pathways." Food Chemistry Advances 2 (2023): 100238.
[3] Chowjarean, Verisa, et al. "Isovitexin increases stem cell properties and protects against PM2. 5 in keratinocytes." in vivo 33.6 (2019): 1833-1841.
[4] Lv, Hongming, et al. "Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways." International journal of biological sciences 12.1 (2016): 72.
[5] Pal, Subhashis, et al. "Oral Administration of Isovitexin, a Naturally Occurring Apigenin Derivative Showed Osteoanabolic Effect in Ovariectomized Mice: A Comparative Study with Teriparatide: S. Pal et al." Calcified Tissue International 111.2 (2022): 196-210.
Isovitexin是从水稻稻壳中提取的一种物质，具有多种生物活性，包括抗炎和抗氧化活性[1]。
在体外实验中，Isovitexin（5-20μM；24h）可保护HaCaT细胞免受H2O2诱导的氧化损伤，并减轻H2O2诱导的细胞凋亡[2]。用10-50μM Isovitexin预处理HaCaT细胞30分钟可完全消除PM2.5诱导的活性氧水平升高[3]。
在体内实验中，Isovitexin（50或100mg/kg; i.p.）预处理可降低脂多糖LPS诱导的急性肺损伤（ALI）小鼠体内肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的生成、活性氧的生成以及髓过氧化物酶（MPO）和丙二醛（MDA）的含量，同时提高超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平，并抑制诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白表达[4]。Isovitexin（5mg/kg；口服）可恢复卵巢切除（OVX）骨质疏松小鼠的骨量和骨小梁（股骨干骺端和第五腰椎/L5）及皮质骨（股骨干）的结构。Isovitexin的口服生物利用度为14.58%[5]。