Isovitexin是从水稻稻壳中提取的一种物质，具有多种生物活性，包括抗炎和抗氧化活性。
Cas No.:38953-85-4 ; 29702-25-8
Sample solution is provided at 25 µL, 10mM.
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Isovitexin extracted from rice hulls of Oryza sativa, possesses various biological activities, including anti-inflammatory and antioxidant activities[1].
In vitro，Isovitexin (5-20μM; 24h) protected HaCaT cells from H2O2-induced oxidative damage, alleviated H2O2-induced apoptosis[2]. Pretreatment HaCaT cells with 10-50μM Isovitexin for 30min completely abolished PM2.5-induced elevation of ROS levels[3].
In vivo, Isovitexin (50 or 100mg/kg; i.p.; once) pretreatment decreased TNF-α and IL-6 production, ROS generation, and MPO and MDA content, while increased SOD and GSH levels and inhibited the protein expression of iNOS and COX-2 in LPS-induced acute lung injury (ALI) mice[4]. Isovitexin (5mg/kg; oral) restored bone mass and architecture of trabecular bones (femur metaphysis and fifth lumbar vertebra/L5) and cortical bones (femur diaphysis) in ovariectomized (OVX) osteopenic mice. Isovitexin has an oral bioavailability of 14.58%[5].
References:
[1] Kang, Jie, et al. "Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities." Food chemistry 128.1 (2011): 152-157.
[2] Lv, Xuechun, et al. "The anti-apoptosis effect of isovitexin on human keratinocytes by regulating miR-98-5p/Bcl-2/Bcl-xL and MAPKs/NF-κB signaling pathways." Food Chemistry Advances 2 (2023): 100238.
[3] Chowjarean, Verisa, et al. "Isovitexin increases stem cell properties and protects against PM2. 5 in keratinocytes." in vivo 33.6 (2019): 1833-1841.
[4] Lv, Hongming, et al. "Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways." International journal of biological sciences 12.1 (2016): 72.
[5] Pal, Subhashis, et al. "Oral Administration of Isovitexin, a Naturally Occurring Apigenin Derivative Showed Osteoanabolic Effect in Ovariectomized Mice: A Comparative Study with Teriparatide: S. Pal et al." Calcified Tissue International 111.2 (2022): 196-210.
Isovitexin是从水稻稻壳中提取的一种物质，具有多种生物活性，包括抗炎和抗氧化活性[1]。
在体外实验中，Isovitexin（5-20μM；24h）可保护HaCaT细胞免受H2O2诱导的氧化损伤，并减轻H2O2诱导的细胞凋亡[2]。用10-50μM Isovitexin预处理HaCaT细胞30分钟可完全消除PM2.5诱导的活性氧水平升高[3]。
在体内实验中，Isovitexin（50或100mg/kg; i.p.）预处理可降低脂多糖LPS诱导的急性肺损伤（ALI）小鼠体内肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的生成、活性氧的生成以及髓过氧化物酶（MPO）和丙二醛（MDA）的含量，同时提高超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平，并抑制诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白表达[4]。Isovitexin（5mg/kg；口服）可恢复卵巢切除（OVX）骨质疏松小鼠的骨量和骨小梁（股骨干骺端和第五腰椎/L5）及皮质骨（股骨干）的结构。Isovitexin的口服生物利用度为14.58%[5]。
| Cell experiment [1]: | |
Cell lines | RAW 264.7 |
Preparation Method | RAW 264.7 cells were plated in 96-well plates (1×104 cells/well) and incubated with various concentrations of Isovitexin (final concentration: 0-200μg/ml) and LPS (2μg/ml) for 24h. In addition, the cells were pretreated with Isovitexin (25 or 50μg/ml) for 1h, followed by the addition of H2O2 (300μM). After 24h, cell viability was determined by MTT assay. |
Reaction Conditions | 0-200μg/ml; 24h. |
Applications | 100μg/ml Isovitexin were not toxic to RAW 264.7 cells, but treatment with 200μg/ml Isovitexin caused significant cytotoxicity. The addition of H2O2 markedly reduced cell viability and induced apoptosis, which were attenuated by Isovitexin exposure at 25 and 50μg/ml. |
| Animal experiment [1]: | |
Animal models | 6-8 weeks old male BALB/c mice |
Preparation Method | Mice were randomly divided into six groups: control (saline), Isovitexin only (100mg/kg, dissolved in 0.5% DMSO), LPS only (0.5mg/kg, dissolved in saline), LPS (0.5mg/kg) + Isovitexin (50 or 100mg/kg), and LPS (0.5mg/kg) + dexamethasone (Dex, 5mg/kg dissolved in saline). Isovitexin (50 or 100mg/kg) or Dex (5mg/kg) were administered intraperitoneally. After exposure to Isovitexin or Dex for 1h, the mice were anesthetized with diethyl ether, and LPS was administered intranasally (i.n.) to induce lung injury. After LPS administration for 12h, the animals were euthanized. |
Dosage form | 50 or 100mg/kg; i.p.; once. |
Applications | Isovitexin pretreatment decreased TNF-α and IL-6 production, ROS generation, and MPO and MDA content, while increased SOD and GSH levels and inhibited the protein expression of iNOS and COX-2 in LPS-induced ALI mice. |
References: | |
| Cas No. | 38953-85-4 ; 29702-25-8 | SDF | |
| 别名 | 异牡荆黄素38953-85-4 | ||
| 化学名 | 5,7-dihydroxy-2-(4-hydroxyphenyl)-6-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]chromen-4-one | ||
| Canonical SMILES | C1=CC(=CC=C1C2=CC(=O)C3=C(C(=C(C=C3O2)O)C4C(C(C(C(O4)CO)O)O)O)O)O | ||
| 分子式 | C21H20O10 | 分子量 | 432.38 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:5): 0.16 mg/ml | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3128 mL | 11.5639 mL | 23.1278 mL |
| 5 mM | 462.6 μL | 2.3128 mL | 4.6256 mL |
| 10 mM | 231.3 μL | 1.1564 mL | 2.3128 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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