GlpBio

IR 783

目录号: GC48378纯度: >95.00%
IR 783是一种近红外（NIR）七甲川菁荧光探针，其最大激发/发射波长分别为633/780nm。
IR 783
Cas No.: 115970-66-6
规格价格库存数量操作
10mg¥105.00现货
1
50mg¥210.00现货
1
100mg¥336.00现货
1
250mg¥672.00现货
1
500mg¥1,092.00现货
1
10mM (in 1mL DMSO)¥116.00现货
1
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产品描述 Description

IR 783 is a near-infrared (NIR) heptamethine cyanine fluorescent probe with excitation/emission maxima of 633/780nm, respectively[1]. IR 783 can be used in combination with anticancer drugs to enhance their targeting and therapeutic efficacy[2]. IR 783 is usually used in research related to for cell imaging and drug delivery[3][4][5].

In vitro, treatment of MDA-MB-231 and MCF-7 breast cancer cells with IR 783 (0-160µM; 0-72h) inhibited the proliferation and migration, promoted mitochondrial fission, and decreased filopodia formation in a dose- and time-dependent manner[6].

In vivo, IR 783 (20mg/kg/day; intravenous injection; 4 weeks) significantly inhibited tumor growth, increased mitochondrial translocation of Drp1, and activated the mitochondrial apoptotic pathway in an MDA-MB-231 xenograft mouse model[7].

References:
[1] Yuan J, Yi X, Yan F, et al. Nearinfrared fluorescence imaging of prostate cancer using heptamethine carbocyanine dyes. Mol Med Rep. 2015;11(2):821-828.
[2] Huang QJ, Liao GC, Zhuang XR, et al. Ras inhibitor farnesylthiosalicylic acid conjugated with IR783 dye exhibits improved tumor-targeting and altered anti-breast cancer mechanisms in mice. Acta Pharmacol Sin. 2022;43(7):1843-1856.
[3] Yang X, Shao C, Wang R, et al. Optical imaging of kidney cancer with novel near infrared heptamethine carbocyanine fluorescent dyes. J Urol. 2013;189(2):702-710.
[4] De Los Reyes-Berbel E, Salto-Gonzalez R, Ortega-Muñoz M, et al. PEI-NIR Heptamethine Cyanine Nanotheranostics for Tumor Targeted Gene Delivery. Bioconjug Chem. 2018;29(8):2561-2575.
[5] Yang X, Shi C, Tong R, et al. Near IR heptamethine cyanine dye-mediated cancer imaging. Clin Cancer Res. 2010;16(10):2833-2844.
[6] Li P, Liu Y, Liu W, et al. IR-783 inhibits breast cancer cell proliferation and migration by inducing mitochondrial fission. Int J Oncol. 2019;55(2):415-424.
[7] Tang Q, Liu W, Zhang Q, et al. Dynamin-related protein 1-mediated mitochondrial fission contributes to IR-783-induced apoptosis in human breast cancer cells. J Cell Mol Med. 2018;22(9):4474-4485.

IR 783是一种近红外（NIR）七甲川菁荧光探针，其最大激发/发射波长分别为633/780nm[1]。IR 783可以与抗癌药物联合使用，以增强其靶向性和疗效[2]。IR 783通常用于细胞成像和药物递送相关研究[3][4][5]。

在体外实验中，IR 783（0-160µM；0-72小时）处理MDA-MB-231和MCF-7乳腺癌细胞，以剂量和时间依赖的方式抑制细胞增殖和迁移，促进线粒体分裂，并减少丝状伪足形成[6]。

在体内实验中，IR 783（20mg/kg/天；静脉注射；4周）显著抑制了MDA-MB-231异种移植小鼠模型中的肿瘤生长，增加了Drp1的线粒体转位，并激活了线粒体凋亡途径[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

MDA-MB-231 and MCF-7 breast cancer cells

Preparation Method

The human breast cancer cell lines MDA-MB-231 and MCF-7 were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). Cells were cultured at 37˚C with 5% CO2 and digested every 2 days by 0.25% trypsin. Cells (2×104 cells/well) were seeded onto 96-well plates and allowed to attach. After treatment with various concentrations of IR 783 (0, 20, 40, 60, 80, 100, 120, 140 and 160µM) for 24h or with 80µM IR 783 for different time intervals (0, 3, 6, 12, 24, 36, 48 and 72h) in a 5% CO2 incubator at 37˚C. The proliferation of cells was determined by MTT assay. Cell cycle analysis was performed by flow cytometry. The migration ability of MDA-MB-231 cells was assessed using a wound healing assay. The morphology of mitochondria was studied using a Tecnai 10 transmission electron microscope.

Reaction Conditions

0-160µM; 0-72h

Applications

Treatment of MDA-MB-231 and MCF-7 breast cancer cells with IR 783 inhibited the proliferation and migration, promoted mitochondrial fission, and decreased filopodia formation in a dose- and time-dependent manner.
Animal experiment [2]:

Animal models

Female nude mice

Preparation Method

Female nude mice (5 weeks old) were used in this experiment. MDA-MB-231 cells were mixed with Matrigel (1:1) and injected subcutaneously into the right hind legs of nude mice. Mice were randomized into 2 groups (n=5). IR 783 (20mg/kg) or an equal volume of vehicle was administered daily by intravenous injection starting 7 days after tumour inoculation. Tumour growth and mice body weights were measured every week, and tumour volume was calculated as (length×width2)/2. After 4 weeks of treatment, mice were killed by cervical dislocation, and tumour tissues were harvested and fixed in 4% paraformaldehyde or frozen at-80°C. Histological, immunohistochemical and immunofluorescence analyses were performed. TdT-mediated dUTP nick-end labelling (TUNEL) analysis was used to detect the dead cells in the tissue samples. Tumour tissues from each group were lysed and subjected to western blot analysis.

Dosage form

20mg/kg/day; intravenous injection; 4 weeks

Applications

IR 783 significantly inhibited tumor growth, increased mitochondrial translocation of Drp1, and activated the mitochondrial apoptotic pathway in an MDA-MB-231 xenograft mouse model.

References:
[1] Li P, Liu Y, Liu W, et al. IR-783 inhibits breast cancer cell proliferation and migration by inducing mitochondrial fission. Int J Oncol. 2019;55(2):415-424.
[2] Tang Q, Liu W, Zhang Q, et al. Dynamin-related protein 1-mediated mitochondrial fission contributes to IR-783-induced apoptosis in human breast cancer cells. J Cell Mol Med. 2018;22(9):4474-4485.

产品文档 Product Documents

Purity:>95.00%Appearance:A solid

化学性质Chemical Properties

CAS 号
115970-66-6
SMILES
ClC1=C(CCC/C1=C\C=C2C(C)(C)C3=CC=CC=C3N\2CCCCS([O-])(=O)=O)/C=C/C4=[N+](CCCCS([O-])(=O)=O)C5=CC=CC=C5C4(C)C.[Na+]
分子式
C38H46ClN2O6S2•Na
分子量
749.4 g/mol
溶解性
DMF: 1 mg/ml,DMSO: 1 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): 1 mg/ml
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol