GlpBio

IPPD-Q

目录号: GC91672纯度: >98.00%同义词: N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone
IPPD-Q是N,N'-取代对苯二胺类化合物（PPD）的氧化产物，消耗二硫苏糖醇（DTT）的氧化潜势值为0.93μM min–1μM–1。
IPPD-Q
Cas No.: 68054-73-9
规格价格库存数量操作
1 mg¥728.00现货
1
5 mg¥3,276.00现货
1
10 mg¥5,824.00现货
1
25 mg¥12,740.00现货
1
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文献被引

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    641, 529–536 (2025)
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产品描述 Description

IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate[1-2]. IPPD-Q has been used in air pollution research and pollutant diagnosis research[3].

In vitro, IPPD-Q exhibits EC50 values of 2.97mg/L (30min) for Aquatic bacterium Vibrio fischeri[4]. IPPD-Q treatment (2.97mg/L) for 30min resulted in a significant decrease in metabolic activity and esterase activity and an increase in the degree of membranocyte damage and oxidative stress in Vibrio fischeri[4]. Treatment of hCMEC-D3 cells with 1.00μM IPPD-Q for 24 hours induced cell apoptosis, endothelial-mesenchymal transition, upregulated Plasminogen activator inhibitor-1 (PAI-1) expression, and triggered blood-brain barrier dysfunction[5].

References:
[1] Wang W, Cao G, Zhang J, et al. p-Phenylenediamine-derived quinones as new contributors to the oxidative potential of fine particulate matter[J]. Environmental Science & Technology Letters, 2022, 9(9): 712-717.
[2] Wang W, Cao G, Zhang J, et al. Beyond substituted p-Phenylenediamine antioxidants: Prevalence of their quinone derivatives in PM2. 5[J]. Environmental Science & Technology, 2022, 56(15): 10629-10637.
[3] Mao T, Liu W, Deng J, et al. p-Phenylenediamines and p-phenylenediamine quinone derivatives in rubber consumer products and typical urban dust: Sources, transformation profiles, and health risks[J]. Environment International, 2024, 192: 109042.
[4] Wang W, Chen Y, Fang J, et al. Toxicity of substituted p-phenylenediamine antioxidants and their derived novel quinones on aquatic bacterium: Acute effects and mechanistic insights[J]. Journal of Hazardous Materials, 2024, 469: 133900.
[5] Han M, Xia K, Xue Y, et al. Emergence of More Potent PPD-Qs beyond 6PPD-Q in Human Blood and Cerebrospinal Fluid[J]. Environmental Science & Technology Letters, 2025.

IPPD-Q是N,N'-取代对苯二胺类化合物（PPD）的氧化产物，消耗二硫苏糖醇（DTT）的氧化潜势值为0.93μM min–1μM–1[1-2]。IPPD-Q已被应用于大气污染研究和污染物溯源诊断研究[3]。

在体外，IPPD-Q对水生细菌费氏弧菌的EC50值（30分钟）为2.97mg/L[4]。IPPD-Q处理（2.97mg/L）30分钟会显著降低费氏弧菌的代谢活性和酯酶活性，同时增加细胞膜损伤程度和氧化应激水平[4]。在人脑血管内皮细胞（hCMEC-D3）中，1.00μM浓度的IPPD-Q处理24小时可诱导细胞凋亡和内皮-间质转化，上调纤溶酶原激活物抑制因子-1（PAI-1）的表达，并引发血脑屏障功能障碍[5]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

 hCMEC-D3 cell

Preparation Method

hCMEC-D3 cells were grown in endothelial cell medium (ECM) which included 5% fetal bovine serum (FBS) and 1% endothelial cell growth supplement along with 1% penicillin/streptomycin solution. Under 37 °C conditions with 5% CO2 humidified atmosphere the cell culture medium was changed every two days until cells reached 90% confluency. For 24 hours cells underwent treatment with different concentrations of IPPD-Q including 0, 0.01, 0.10, and 1.00μM. Collect cells from various exposure groups for protein expression analysis using Western blotting assays. Assess whether atorvastatin at a concentration of 0.30μM could modify the cellular effects of IPPD-Q when cells received both treatments simultaneously. The vehicle control that consisted of 0.1% DMSO (v/v) showed negligible effects after its evaluation process and thus became the designated exposure group 0.

Reaction Conditions

 0, 0.01, 0.10, and 1.00μM; 24h

Applications

 IPPD-Q can increase the level of TNF-α, induce cell apoptosis, up-regulate PAI-1 and lead to endothelial-to-mesenchymal transition of hCMEC-D3 cells, resulting in endothelial cell dysfunction.

References:
[1] Han M, Xia K, Xue Y, et al. Emergence of More Potent PPD-Qs beyond 6PPD-Q in Human Blood and Cerebrospinal Fluid[J]. Environmental Science & Technology Letters, 2025.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
68054-73-9
同义词
N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone
分子式
C15H16N2O2
分子量
256.3 g/mol
溶解性
Acetonitrile: Slightly soluble: 0.1-1 mg/ml,DMSO: Sparingly soluble: 1- 10 mg/ml
保存条件
-20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol