Ioversol (MP-328)

Cell experiment:

Cell viability is assessed by using the MTT uptake assay. The conversion of MTT, a tetrazolium salt, into formazan depends on the activity of a group of mitochondrial dehydrogenases and, thus, is an indicator of cell metabolic activity. The color of MTT tetrazole salt is measured with a spectrophotometer at the wavelength of 570 nm. Briefly, fresh MTT solution (10 mg/mL in 1× PBS) isadded (1:20 volume of medium) after removing the medium and the cells are incubated for 1.5 h. The cells are lysed and purple formazan dissolved into the solution by overnight incubation with MTT lysis buffer. The results are expressed as the percentage of undamaged control cells, assuming the absorbance of control wells as 100%.

References:

[1]. Yang D, et al. Selective inhibition of the reverse mode of Na(+)/Ca(2+) exchanger attenuates contrast-induced cell injury. Am J Nephrol. 2013;37(3):264-73. doi: 10.1159/000348526. Epub 2013 Mar 13.
[2]. Heyman SN, et al. Effects of ioversol versus iothalamate on endothelin release and radiocontrast nephropathy. Invest Radiol. 1993 Apr;28(4):313-8.