Influenza Hemagglutinin (HA) Peptide is a synthetic peptide with the amino acid sequence Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala (YPYDVPDYA), derived from residues 98-106 of the HA1 subunit of the human influenza virus hemagglutinin (HA) protein[1]. Influenza Hemagglutinin (HA) Peptide commonly used in immunoprecipitation (IP) and affinity purification experiments as a competitive elution agent. By adding it in excess, Influenza Hemagglutinin (HA) Peptide allows for the gentle elution of HA-tagged fusion proteins in their native state, avoiding the damage to protein activity caused by harsh conditions such as low pH or denaturants[2, 3]. Influenza Hemagglutinin (HA) Peptide can also be used to isolate HA-tagged fusion proteins from monoclonal anti-HA agarose affinity columns[4].
References:
[1] Wagner E, Plank C, Zatloukal K, et al. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle[J]. Proceedings of the National Academy of Sciences, 1992, 89(17): 7934-7938.
[2] Zhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification[J]. Journal of analytical methods in chemistry, 2013, 2013(1): 581093.
[3] Shariat-Panahi A. Evaluating Chemical Crosslinking as a Tool for Enhancing DNA Damage Repair Interactome Analysis[J]. 2022.
[4] Panwar P, Jhala D, Tamrakar A, et al. Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination[J]. Protein Expression and Purification, 2024, 223: 106541.
Influenza Hemagglutinin (HA) Peptide是一种人工合成肽，其氨基酸序列为 Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala（YPYDVPDYA），来源于人流感病毒血凝素（HA）蛋白HA1亚基的第98-106位残基[1]。Influenza Hemagglutinin (HA) Peptide通常用于免疫沉淀（IP）和亲和纯化实验中，作为竞争性洗脱剂，通过过量添加，将HA标签融合蛋白以天然形式温和地洗脱下来，避免了使用低pH值或变性剂等苛刻条件对蛋白活性的损害[2, 3]。Influenza Hemagglutinin (HA) Peptide也可用于从单克隆抗-HA琼脂糖亲和柱中分离带HA标签的融合蛋白[4]。