ICG-carboxylic acid is a near-infrared (NIR) fluorescent probe based on indocyanine green (ICG), characterized by good water solubility, low toxicity, and stability[1]. As a carboxylated form of ICG, ICG-carboxylic acid has an absorption/emission wavelength of approximately 785/800nm[2]. The carboxylic acid group is activated by the EDC/NHS reaction, making ICG-carboxylic acid readily conjugatable with other biomolecules, such as proteins, peptides, and nucleic acids[3]. ICG-carboxylic acid is commonly used in biomedical research fields, including tissue imaging, photothermal therapy, tumor detection, and drug delivery systems[4-7].
References:
[1] Taylor, J. S., Zeki, J., Ikegaki, N., Chen, L. L., & Chiu, B. (2018). Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction. Journal of pediatric surgery, 53(12), 2475–2479.
[2] Mačianskienė, R., Almanaitytė, M., Treinys, R., Navalinskas, A., Benetis, R., & Jurevičius, J. (2017). Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Scientific reports, 7(1), 7983.
[3] Wang, P., Wang, X., Luo, Q., Li, Y., Lin, X., Fan, L., Zhang, Y., Liu, J., & Liu, X. (2019). Fabrication of Red Blood Cell-Based Multimodal Theranostic Probes for Second Near-Infrared Window Fluorescence Imaging-Guided Tumor Surgery and Photodynamic Therapy. Theranostics, 9(2), 369–380.
[4] Kaplan-Marans, E., Fulla, J., Tomer, N., Bilal, K., & Palese, M. (2019). Indocyanine Green (ICG) in Urologic Surgery. Urology, 132, 10–17.
[5] Chen, Z., Zhao, P., Luo, Z., Zheng, M., Tian, H., Gong, P., Gao, G., Pan, H., Liu, L., Ma, A., Cui, H., Ma, Y., & Cai, L. (2016). Cancer Cell Membrane-Biomimetic Nanoparticles for Homologous-Targeting Dual-Modal Imaging and Photothermal Therapy. ACS nano, 10(11), 10049–10057.
[6] Shao, C., Li, Z., Zhang, C., Zhang, W., He, R., Xu, J., & Cai, Y. (2022). Optical diagnostic imaging and therapy for thyroid cancer. Materials today. Bio, 17, 100441.
[7] Lu, K., Pan, X., Zheng, J., Cheng, D., Zheng, L., & Zhang, X. (2024). Theranostic nanoparticles ZIF-8@ICG for pH/NIR-responsive drug-release and NIR-guided chemo-phototherapy against non-small-cell lung cancer. Journal of materials science. Materials in medicine, 35(1), 32.
ICG-carboxylic acid是一种基于吲哚菁绿（ICG）的近红外（NIR）荧光探针，具有良好的水溶性、低毒性及稳定性[1]。ICG-carboxylic acid是ICG的羧基化形式，吸收/发射波长约785/800nm[2]。羧酸基团通过EDC/NHS 反应活化后使ICG-carboxylic acid易于与其他生物分子（如蛋白质、多肽、核酸等）进行偶联反应[3]。ICG-carboxylic acid通常用于组织成像、光热治疗、肿瘤检测以及药物递送系统等生物医学研究领域[4-7]。