T36 is a low molecular weight drophobic tag that promotes the degradation of fusion protein and pseudokinase Her3[1][2][3].
T36 (10 µM；24 h) 降低表达 GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 融合蛋白的 HEK293 细胞中 GFP-HaloTag2/Fz4-HaloTag2 的表达[1]。
T36 (10 µM；2 h 或 48 h) 降低了表达 HA-EGFP-HaloTag2 的 HEK293 Flp-In 细胞中 EGFP 的表达，加强了 Hsp70 与 HA-EGFP-HaloTag2 融合蛋白的结合[3]。
T36 (50 nM；24 h) 降低了表达 HA-EGFP-HaloTag2 的 HEK293 Flp-In 细胞中 EGFP-HaloTag2 的含量[3]。
T36 (1 µM；4 d) 降低了表达 HA-HaloTag2-HRasG12V 的 NIH-3T3 细胞中 HA-HaloTag2-HRasG12V 的表达[3]。
Western Blot Analysis[1][3]
| Cell Line: | HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/HA-EGFP-HaloTag2 or NIH-3T3 cells expressing HA-HaloTag2-HRasG12V. |
| Concentration: | 1 µM or 10 µM. |
| Incubation Time: | 2 h, 24 h or 4 days. |
| Result: | Decreased the expression of fusion proteins and increased the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein. |
[1]. Tae HS, et al. Identification of drophobic tags for the degradation of stabilized proteins. Chembiochem. 2012 Mar 5;13(4):538-41.
[2]. Rubner S, et al. Poloxin-2HT+: changing the drophobic tag of Poloxin-2HT increases Plk1 degradation and apoptosis induction in tumor cells. Org Biomol Chem. 2019 Mar 20;17(12):3113-3117.
[3]. Neklesa TK, et al. A bidirectional system for the dynamic small molecule control of intracellular fusion proteins. ACS Chem Biol. 2013 Oct 18;8(10):2293-2300.