Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. Hyaluronidase can reduce tissue viscosity and increase tissue permeability through the hydrolysis of hyaluronic acid. Hyaluronidase is used in research related to drug diffusion assistance and the management of complications in medical aesthetics[1-4].
In vitro, Hyaluronidase (400μg/ml) was used to treat 3T3L1 adipocyte cell lines and mouse primary preadipocytes (from subcutaneous, visceral, or brown adipose tissue) for 8 days. Hyaluronidase significantly inhibited adipocyte differentiation and reduced lipid accumulation[5]. Hyaluronidase (400μg/ml) was applied to LiSa-2 liposarcoma cells for 24 hours. Hyaluronidase significantly increased cellular glucose uptake and lactate production, while enhancing plasma membrane GLUT1 localization and reducing TXNIP levels[6].
In vivo, Hyaluronidase (1U; 10μl) was administered via stereotactic intracranial injection in a controlled cortical impact traumatic brain injury model using C57BL/6J mice. Hyaluronidase significantly reduced brain edema after traumatic brain injury[7]. Hyaluronidase (12mg/kg) was intravenously injected into C57BL/6 mice for 30 minutes. Hyaluronidase significantly decreased the thickness of the retinal arteriolar glycocalyx and increased retinal microvascular permeability[8].
References:
[1] Yuan J, Hou B, Guo K, et al. Tumor-derived exosomal hyaluronidase 1 induced M2 macrophage polarization and promoted esophageal cancer progression. Exp Cell Res. 2024 Jun 1;439(1):113963.
[2] Shakouri A, Parvan R, Adljouy N, et al. Purification of hyaluronidase as an anticancer agent inhibiting CD44. Biomed Chromatogr. 2020 Jan;34(1):e4709.
[3] Tolwinski NS, Kano-Sueoka T. Hyaluronidase generates a single-cell suspension from cultured mouse lung epithelial cells. Biotechniques. 1997 May;22(5):856-8, 860.
[4] Meuwese MC, Broekhuizen LN, Kuikhoven M, et al. Endothelial surface layer degradation by chronic hyaluronidase infusion induces proteinuria in apolipoprotein E-deficient mice. PLoS One. 2010 Dec 8;5(12):e14262.
[5] Drygalski K, Higos R, Merabtene F, et al. Extracellular matrix hyaluronan modulates fat cell differentiation and primary cilia dynamics. Biochim Biophys Acta Mol Cell Biol Lipids. 2024 May;1869(4):159470.
[6] Sullivan WJ, Mullen PJ, Schmid EW, et al. Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization. Cell. 2018 Sep 20;175(1):117-132.e21.
[7] Washington PM, Lee C, Dwyer MKR, et al. Hyaluronidase reduced edema after experimental traumatic brain injury. J Cereb Blood Flow Metab. 2020 Oct;40(10):2026-2037.
[8] Leskova W, Pickett H, Eshaq RS, et al. Effect of diabetes and hyaluronidase on the retinal endothelial glycocalyx in mice. Exp Eye Res. 2019 Feb;179:125-131.
Hyaluronidase是一种能水解透明质酸的酶，Hyaluronidase可通过水解透明质酸来降低组织粘度并增加组织通透性。Hyaluronidase可用于药物扩散辅助和医美并发症处理的相关研究[1-4]。
在体外，Hyaluronidase（400μg/ml）处理3T3L1脂肪细胞系、小鼠原代前脂肪细胞（来自皮下、内脏或棕色脂肪组织）8天。Hyaluronidase显著抑制脂肪细胞分化，减少脂质积累[5]。Hyaluronidase（400μg/ml）处理LiSa-2脂肪肉瘤细胞24小时。Hyaluronidase显著增加细胞的葡萄糖摄取和乳酸产生，同时增加质膜GLUT1定位并降低TXNIP水平[6]。
在体内，Hyaluronidase（1U；10μl）通过立体定位颅内注射于控制性皮质撞击创伤性脑损伤模型C57BL/6J小鼠的。Hyaluronidase显著减少了创伤性脑损伤后的脑水肿[7]。Hyaluronidase（12mg/kg）静脉注射于C57BL/6小鼠30分钟。Hyaluronidase显著减少了视网膜小动脉糖萼的厚度，同时增加了视网膜微血管的通透性[8]。