The activation of T cells typically requires TCR/CD3 signaling and CD28 co stimulatory signaling. The TCR/CD3 signal binds to antigen-presenting molecules on antigen-presenting cells through T cell receptors (TCRs) on the surface of T cells, initiating preliminary activation signals. The CD28 co stimulatory signal binds to B7 molecules (such as CD80 and CD86) on antigen-presenting cells through the surface of T cells, providing a second signal that enhances T cell activation, proliferation, and survival.
The activation of human CD3/CD28 T cells by magnetic beads is based on two important co stimulatory signals, CD3 and CD28. Anti-CD3 and Anti-CD28 antibodies coupled to the surface of the magnetic beads can activate TCR and CD28 co stimulatory signals, respectively, without relying on feeder layer cells (antigen-presenting cells) or antigens to activate T cells quickly and easily. During the cell culture process, T cell expansion can be stimulated by adding recombinant human IL-2. After cell activation or expansion, magnetic beads can be removed through a magnetic rack.
This product is suitable for T cell activation of peripheral blood mononuclear cells (PBMCs) or T cell subsets (such as CD3<sup+<sup="">T cells, CD4<sup+<sup="">T cells, and CD8<sup+<sup="">T cells).
T 细胞的激活通常需要 TCR/CD3 信号和 CD28 共刺激信号。TCR/CD3 信号通过 T 细胞表面的 T 细胞受体 (TCR) 与抗原递呈细胞上的抗原呈递分子结合，启动初步的激活信号。CD28 共刺激信号通过 T 细胞表面的 CD28 与抗原递呈细胞上的 B7 分子（如 CD80、CD86）结合，提供第二信号，增强 T 细胞的激活、增殖及存活。
人 CD3/CD28 T 细胞激活磁珠基于 CD3 和 CD28 两种重要的共刺激信号，磁珠表面偶联的 Anti-CD3 和 Anti-CD28 抗体可分别激活 TCR 和 CD28 共刺激信号，无需依赖饲养层细胞（抗原递呈细胞）或抗原即可简单快速地激活 T 细胞。细胞培养过程中可通过添加重组人 IL-2 来刺激 T 细胞扩增。细胞激活或扩增后，可通过磁力架去除磁珠。
本品适用于外周血单核细胞 (PBMC) 或 T 细胞亚群（如 CD3<sup+< sup=""> T 细胞、CD4<sup+< sup=""> T 细胞及 CD8<sup+< sup=""> T 细胞）的 T 细胞激活。