Homopropargylglycine is a Methionine analogue. Homopropargylglycine is incorporated at a suitable rate into newly synthesized proteins in a variety of systems, including mammalian cell culture. Homopropargylglycine can be used to identify old and new protein populations[1].
Homopropargylglycine enables Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) of a small sample of the proteins being synthesized in Arabidopsis plants or cell cultures, facilitating their click-chemistry enrichment for analysis[1].
[1]. Tivendale ND, Millar AH, et al. In vivo homopropargylglycine incorporation enables sampling, isolation and characterization of nascent proteins from Arabidopsis thaliana. Plant J. 2021 Aug;107(4):1260-1276.