Green CMFDA, as a non-terminal, non-fluorescent probe, can be cleaved by non-specific esterases common to living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope[1]. CMFDA is excited with a monochromator at 492 nm and is emissed at 548 nm[2].
In vitro, there was a a positive chondrogenic process of hBM-MSC labeled with 10 μM CellTracker? Green CMFDA, indicating these cells could differentiate into chondrogenic cells similarly to controlled non-labeled cells[3]. In vitro experiment it shown that 4 weeks after grafting stem cells, the green fluorescence of the originally CMFDA-labeled (10 μM) bladder epithelia was still detectable in all of the resulting glandular prostate epithelium[4].
References:
[1] Bernhard, et al. Comparison of two methods to identify live benthic foraminifera: a test between Rose Bengal and CellTracker Green with implications for stable isotope paleoreconstructions. Paleoceanography. 2006;21.
[2] Trontelj K, et al. Cell electrofusion visualized with fluorescence microscopy. J Vis Exp. 2010 Jul 1;(41):1991.
[3] Andrzejewska A, et al. Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity. Stem Cell Res Ther. 2019 Jun 25;10(1):187.
[4] Li X, et al. Urothelial transdifferentiation to prostate epithelia is mediated by paracrine TGF-beta signaling. Differentiation. 2009 Jan;77(1):95-102.
绿色 CMFDA 作为一种非末端、非荧光探针,可以被活细胞常见的非特异性酯酶切割,产生荧光化合物荧光素,使用荧光显微镜可见[1]。 CMFDA 在 492 nm 处用单色器激发,并在 548 nm 处发射[2]。
在体外,用 10 μM CellTracker™ Green CMFDA 标记的 hBM-MSC 有一个阳性软骨形成过程,表明这些细胞可以像对照的未标记细胞一样分化成软骨形成细胞[3] .体外实验表明,移植干细胞 4 周后,在所有生成的腺体前列腺上皮细胞中仍可检测到最初 CMFDA 标记的 (10 μM) 膀胱上皮细胞的绿色荧光[4]。