This product is derived from oyster glycogen. It is free of DNase, RNase and protease, which can be used as a carrier to increase the recovery of nucleic acids from diluted solutions by alcohol precipitation.
As a precipitant for nucleic acids, glycogen works better than tRNA or sonicated DNA in most cases. Since glycogen does not contain DNA and RNA, the nucleic acids precipitated with glycogen is more suitable for subsequent PCR, RT-PCR, and endonuclease reactions.
It was reported that precipitation of ligation products with glycogen has no interference with subsequent bacterial transformations. Glycogen of 0.001mg/ml will not inhibit TdT, glycogen of no more than 2mg/ml will not affect the activity of reverse transcriptase, and 0.02mg/ml glycogen will not inhibit T4 RNA ligase. However, glycogen interferes with DNA-protein interactions.
Usually, 1μl of Glycogen (20mg/ml) can precipitate at least picogram (pg) of DNA or RNA from 1ml of solution. The 0.5ml, 2ml and 10ml packages of this product are sufficient to precipitate at least 500, 2000 and 10000 DNA or RNA samples in conventional quantities, respectively.
Precautions：
Usually, 1μl of Glycogen (20mg/ml) is sufficient for each sample. If glycogen may interfere with subsequent reactions, the amount of glycogen can be appropriately reduced, or tRNA can be used as a precipitant instead.
本产品为分子生物学级Glycogen(糖原)，来源为牡蛎糖原，即oyster glycogen。不含DNase，不含RNase，不含蛋白酶，可以用作沉淀DNA或RNA的辅助沉淀剂(used as a carrier for the precipitation of DNA or RNA)。
本产品经过了严格的DNase, RNase & Protease free质检，确保没有DNase、RNase和蛋白酶污染。
作为DNA或RNA的辅助沉淀剂，大多数情况下glycogen比tRNA或超声处理过的DNA效果更好。由于glycogen中不含DNA和RNA，因此用glycogen作为辅助沉淀剂沉淀下来的核酸更适合于后续的PCR、RT-PCR以及内切酶等核酸酶反应。而tRNA或超声处理过的DNA作为辅助沉淀剂有时会干扰PCR、RT-PCR以及内切酶等核酸酶反应。
据文献报道，连接反应产物用glycogen沉淀后对于后续的细菌转化没有干扰，0.001mg/ml glycogen不会抑制TdT，浓度不大于2mg/ml的glycogen不会影响反转录酶的活力，0.02mg/ml glycogen不会抑制T4 RNA ligase。Glycogen会干扰DNA和蛋白的相互作用。
通常1微升Glycogen (20mg/ml)即可把少至皮克(pg)级的DNA或RNA从1毫升的溶液体系中沉淀出来。0.5ml、2ml、10ml包装的本产品至少足够沉淀500个，2000个，10000个常规量的DNA或RNA样品。
注意事项：
通常每个样品加入1微升Glycogen (20mg/ml)即可，对于已知糖原可能对后续反应有干扰的情况，可以适当减少糖原用量，或使用tRNA等作为辅助沉淀剂。