Glucose oxidase(100-250u/mg)

Glucose oxidase(100-250u/mg) is an oxidoreductase derived from Aspergillus niger. When oxygen and glucose are present, Glucose oxidase (100-250u/mg) can exhibit antibacterial activity ^{[1] [2] [3]}.

Glucose oxidase (100-250u/mg) is a yellow amorphous powder derived from Aspergillus niger. Its enzyme commission number is EC 1.1.3.4. Under specific conditions (pH 5.7, 37°C), one unit of glucose oxidase can oxidize 1μM glucose per minute. The molecular weight of the enzyme is about 150 kDa as determined by SDS-PAGE. Its isoelectric point is 5.2, showing certain charge properties. For the substrate D-glucose, the Michaelis constant of the enzyme is 4.9×10^-2 M. Actual measurements show that the pH stability of the product is 5.5~7.5 (25°C, 18 hours) and the thermal stability is <40°C (pH 5.7, 60 minutes). It is worth noting that metal ions such as Cu²⁺, Fe³⁺, Mg²⁺ and Mn²⁺ have an inhibitory effect on glucose oxidase (100-250u/mg). Glucose oxidase(100-250u/mg) specifically binds to β-D-pyranose glucose and does not act on α-D-glucose. In the presence of air and glucose, Glucose oxidase(100-250u/mg) catalyzes the oxidation of β-D-glucose to D-glucono-1,5-lactone, which is then hydrolyzed to gluconic acid^[4]. Glucose oxidase(100-250u/mg) is widely used in blood glucose monitoring, free glucose detection in plant raw materials and food industries, and food preservation. It also has many applications in biotechnology, usually for enzyme assays in biochemistry, biosensors in nanotechnology^[5].

References:
[1].Wong C M, Wong K H, Chen X D. Glucose oxidase: natural occurrence, function, properties and industrial applications[J]. Applied microbiology and biotechnology, 2008, 78: 927-938.
[2].Broom W A, Coulthard C E, Gurd M R, et al. Some pharmacological and chemotherapeutic properties of notatin[J]. British journal of pharmacology and chemotherapy, 1946, 1(4): 225.
 [3].Ghoshdastider U, Wu R, Trzaskowski B, et al. Molecular effects of encapsulation of glucose oxidase dimer by graphene[J]. RSC Advances, 2015, 5(18): 13570-13578.
[4]. Bauer J A, Zámocká M, Majtán J, et al. Glucose oxidase, an enzyme “Ferrari”: Its structure, function, production and properties in the light of various industrial and biotechnological applications[J]. Biomolecules, 2022, 12(3): 472.
[5].Heller A, Ulstrup J. Detlev Müller’s Discovery of Glucose Oxidase in 1925[J]. Analytical Chemistry, 2021, 93(18): 7148-7149.

Glucose oxidase(100-250u/mg)是一种来源于黑曲霉的氧化还原酶，当存在氧气和葡萄糖时，Glucose oxidase(100-250u/mg)可表现出抗菌活性^{[1] [2] [3]}。

Glucose oxidase(100-250u/mg)时一种黄色无定形粉末状，源自黑曲霉（Aspergillus niger）。其酶委员会编号为EC 1.1.3.4，在特定条件下（pH 5.7，37℃），一个单位的Glucose oxidase每分钟能氧化1μM的葡萄糖。通过SDS-PAGE测定，该酶的分子量约为150 kDa。它的等电点为5.2，显示出一定的电荷性质。对于底物D-葡萄糖，该酶的米氏常数为4.9×10^-2 M。经实际测量显示，该产品pH稳定性为5.5~7.5（25℃，18小时），热稳定性为<40℃（pH 5.7，60分钟）。值得注意的是，Cu²⁺、Fe³⁺、Mg²⁺和Mn²⁺等金属离子对Glucose oxidase(100-250u/mg)具有抑制作用。Glucose oxidase(100-250u/mg)与 β-D-吡喃葡萄糖特异性结合，不作用于 α-D-葡萄糖。在空气与葡萄糖同时存在时，Glucose oxidase(100-250u/mg)催化 β-D-葡萄糖氧化成 D-葡萄糖酸-1,5-内酯，然后水解成葡萄糖酸^[4]。Glucose oxidase(100-250u/mg)广泛用于血糖监测、植物原料和食品工业中的游离葡萄糖检测和食品保鲜等，也用于生物技术中的酶测定和纳米技术中的生物传感器^[5]。