Girard’s reagent P-d5 is intended for use as an internal standard for the quantification of Girard’s reagent P by GC- or LC-MS. Girard’s reagent P is a cationic hydrazine reagent.1 It has been used as a derivatization reagent to quantify glycans, oxysterols, and 5-methylcytosine derivatives.2,3,4
1.Wheeler, O.H.The Girard ReagentsChem. Rev.62(3)205-221(1962) 2.Wang, C., Wu, Z., Yuan, J., et al.Simplified quantitative glycomics using the stable isotope label Girard’s reagent P by electrospray ionization mass spectrometryJ. Proteme Res.13(2)372-384(2014) 3.Griffiths, W.J., Abdel-Khalik, J., Crick, P.J., et al.Sterols and oxysterols in plasma from Smith-Lemli-Opitz syndrome patientsJ. Steroid Biochem. Mol. Biol.16977-87(2017) 4.Tang, Y., Xiong, J., Jiang, H.-P., et al.Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysisAnal. Chem.86(15)7764-7772(2014)