The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli [1][2][3][4][5][6][7].
References:
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绿色荧光蛋白 (GFP) 是一种在蓝紫光范围内曝光时会发出绿色荧光的蛋白质，发现于包括 Aequorea victoria，珊瑚，海葵，浮游动物，桡足类和文昌鱼在内的生物中。GFP 蛋白 (Aequorea victoria) 的主要激发峰波长在 395 nm ，次要激发峰波长在 475 nm，而其发射峰波长在 509 nm，位于可见光谱的较低绿色部分。GFP 蛋白作为能量转移受体，积极将蛋白质 aequorin 的蓝色化学发光通过能量转移转化为绿色荧光光，被用作报告基因的表达。GFP 蛋白, Aequorea victoria (His) 是重组的水母 Aequorea victoria GFP 蛋白，带有 N-6*His 标签，由 238 个氨基酸组成，由 E. coli 表达。