MTS Reagent, cell proliferation assay reagent is a novel water-soluble tetrazolium salt compound. MTS Reagent can be reduced by dehydrogenases in the mitochondria of living cells to generate a water-soluble formazan dye. The absorbance of this product at 490nm is proportional to the number and metabolic activity of viable cells in the culture system. MTS Reagent can be used for rapid and highly sensitive detection of cell proliferation, cytotoxicity, and chemical sensitivity[1-4].
References:
[1] Kabakov AE, Gabai VL. Cell Death and Survival Assays. Methods Mol Biol. 2018;1709:107-127.
[2] Zheng X, Chang F, Rong Y, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker, promotes proliferation and invasion of triple negative breast cancer. Exp Mol Pathol. 2022 Apr;125:104751.
[3] Soto-Gamez A, Wang Y, Zhou X, et al. Enhanced extrinsic apoptosis of therapy-induced senescent cancer cells using a death receptor 5 (DR5) selective agonist. Cancer Lett. 2022 Jan 28;525:67-75.
[4] Singh RR, Kulshreshtha R, Popat A. Ultrasmall nanoparticles for co-delivery of antisense oligonucleotides targeting miR-21 and miR-210 to treat glioblastoma. J Nanobiotechnology. 2025 Jul 2;23(1):482.
MTS Reagent, cell proliferation assay reagent是一种新型的、具有水溶性的四氮唑盐类化合物。MTS Reagent可被活细胞线粒体内的脱氢酶还原生成水溶性的甲臜染料，该产物在490nm处的吸光度值与培养体系中活细胞的数量和代谢活性成正比，MTS Reagent可用于细胞增殖、细胞毒性以及化学敏感性的快速高灵敏度检测[1-4]。