Genistein-d4 is intended for use as an internal standard for the quantification of genistein by GC- or LC-MS. Genistein is an isoflavonoid phytoestrogen that has been found in soybeans (G. max/S. hispida) and has kinase inhibitory, anticancer, pro-cancer, hepatoprotective, and antiviral properties.1 It inhibits the tyrosine kinases EGFR, pp50v-Src, and pp110gag-fes (IC50 = 6, 7-8, and 6.5 µg/ml, respectively) and decreases EGF-induced serine, threonine, and tyrosine phosphorylation of EGFR in A431 cells when used at a concentration of 20 µg/ml.2 Genistein inhibits proliferation and induces apoptosis in a variety of cancer cells, including Bel 7402 hepatocellular carcinoma cells when used at a concentration of 10 µg/ml.1,3 It reduces tumor invasion and angiogenesis in a Bel 7402 mouse subrenal capsule xenograft model when administered at a dose of 50 mg/kg per day.3 However, when administered at the same dose on postnatal days 1-5, genistein increases the incidence of uterine adenocarcinoma in a mouse model of cancer induced by the estrogen receptor agonist diethylstilbestrol .4 It reduces lipid accumulation and inflammation in the liver of ovariectomized (OVX) and non-OVX female rats in a model of high-fat high-fructose diet-induced nonalcoholic hepatosteatosis (NASH) when administered at a dose of 16 mg/kg per day.5 Genistein (10 µM) also inhibits HIV-1 DNA synthesis in resting CD4+ T cells.6
1.Spagnuolo, C., Russo, G.L., Orhan, I.E., et al.Genistein and cancer: Current status, challenges, and future directionsAdv. Nutr.6(4)408-419(2015) 2.Akiyama, T., Ishida, J., Nakagawa, S., et al.Genistein, a specific inhibitor of tyrosine-specific protein kinasesJ. Biol. Chem.262(1)5592-5595(1987) 3.Gu, Y., Zhu, C.-F., Iwamoto, H., et al.Genistein inhibits invasive potential of human hepatocellular carcinoma by altering cell cycle, apoptosis, and angiogenesisWorld J. Gastroenterol.11(41)6512-6517(2005) 4.Newbold, R.R., Banks, E.P., Bullock, B., et al.Uterine adenocarcinoma in mice treated neonatally with genisteinCancer Res.614325-4328(2001) 5.Pummoung, S., Werawatganon, D., Klaikeaw, N., et al.Genistein-attenuated hepatic steatosis and inflammation in nonalcoholic steatohepatitis with bilateral ovariectomized ratsPharmacogn. Mag.14(55)20-24(2018) 6.Guo, J., Xu, X., Rasheed, T.K., et al.Genistein interferes with SDF-1- and HIV-mediated actin dynamics and inhibits HIV infection of resting CD4 T cellsRetrovirology1062(2013)