ROS fluorescent probe DHE is the commonly used fluorescent dyes to measure ROS in cells and tissues at 480nm wavelength excitation and 610nm wavelength emission [1]. ROS fluorescent probe DHE can be rapidly absorbed by cells. After specifically reacting with superoxide free radicals, it will generate a red fluorescent product, 2-hydroxyethidium[2]. ROS fluorescent probe DHE has been widely used in the development of novel high-performance liquid chromatography (HPLC) methods for the rapid detection of superoxide in intact cells and tissues[3].
References:
[1] Kumar R, Gullapalli R R. High throughput screening assessment of reactive oxygen species (ROS) generation using Dihydroethidium (DHE) fluorescence dye[J]. Journal of visualized experiments: JoVE, 2024 (203): 10.3791/66238.
[2] Zhu L, Lu M, Yin X. Ultrasensitive determination of intracellular superoxide in individual HepG2 cells by microfluidic chip electrophoresis[J]. Talanta, 2008, 75(5): 1227-1233.
[3] Fink B, Laude K, McCann L, et al. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay[J]. American Journal of Physiology-Cell Physiology, 2004, 287(4): C895-C902.
ROS fluorescent probe DHE是用于检测细胞和组织中活性氧的常用荧光染料，激发波长为480nm，发射波长为610nm[1]。ROS fluorescent probe DHE能被细胞快速摄取，与超氧自由基特异性反应后生成红色荧光产物2-羟基乙锭[2]。ROS fluorescent probe DHE已广泛应用于新型高效液相色谱方法的开发，用于快速检测完整细胞和组织中的超氧阴离子[3]。