β-Amyrin是一种天然存在的五环三萜类化合物，具有抗炎、抗菌、抗氧化作用。β-Amyrin能够有效阻断β-淀粉样蛋白诱导的长时程增强损伤。
Cas No.:559-70-6
Sample solution is provided at 25 µL, 10mM.
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β-Amyrin is a naturally occurring pentacyclic triterpenoid compound that possesses anti-inflammatory, antibacterial, and antioxidant activities[1-2]. β-Amyrin can be used in research related to Alzheimer's disease, cancer, obesity, atherosclerosis, and type II diabetes[3-4].
In vitro, Hep-G2 hepatocellular carcinoma cells were treated with β-Amyrin (0-100μM) for 24 hours. β-Amyrin significantly induced apoptosis and caused cell cycle arrest at the G2/M phase, while simultaneously activating the JNK and p38 signaling pathways[5]. Escherichia coli (E. coli) cells were treated with β-Amyrin (5μM) for 2 hours. β-Amyrin induced apoptosis-like death in the cells by triggering the accumulation of reactive oxygen species (ROS) and glutathione dysfunction, which led to cell membrane depolarization, resulting in DNA fragmentation and phosphatidylserine externalization[6].
In vivo, an amyloid-β (Aβ)-injected Alzheimer's disease (AD) mouse model was orally administered β-Amyrin (4mg/kg) daily from day 1 to day 5 after Aβ (0.5mg; 35-40μl; or 1mg; 70-80μl) injection. β-Amyrin significantly ameliorated the Aβ-induced impairments in object recognition memory and passive avoidance memory[7]. ICR mice with scopolamine-induced memory impairment were orally administered β-Amyrin (4mg/kg) 60 minutes before training. β-Amyrin significantly ameliorated the scopolamine-induced memory impairment in the passive avoidance task[8].
References:
[1] Viet TD, Xuan TD, Anh H. α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules. 2021 Nov 29;26(23):7248.
[2] Kwun MS, Lee HJ, Lee DG. β-amyrin-induced apoptosis in Candida albicans triggered by calcium. Fungal Biol. 2021 Aug;125(8):630-636.
[3] Oliveira RC, Bandeira PN, Lemos TLG, et al. In silico and in vitro evaluation of efflux pumps inhibition of α,β-amyrin. J Biomol Struct Dyn. 2022;40(23):12785-12799.
[4] Mus AA, Goh LPW, Marbawi H, et al. The Biosynthesis and Medicinal Properties of Taraxerol. Biomedicines. 2022 Mar 30;10(4):807.
[5] Wen S, Gu D, Zeng H. Antitumor effects of beta-amyrin in Hep-G2 liver carcinoma cells are mediated via apoptosis induction, cell cycle disruption and activation of JNK and P38 signalling pathways. J BUON. 2018 Jul-Aug;23(4):965-970.
[6] Han G, Lee DG. Antibacterial Mode of Action of β-Amyrin Promotes Apoptosis-Like Death in Escherichia coli by Producing Reactive Oxygen Species. J Microbiol Biotechnol. 2022 Dec 28;32(12):1547-1552.
[7] Park HJ, Kwon H, Lee JH, et al. β-Amyrin Ameliorates Alzheimer's Disease-Like Aberrant Synaptic Plasticity in the Mouse Hippocampus. Biomol Ther (Seoul). 2020 Jan 1;28(1):74-82.
[8] Park SJ, Ahn YJ, Oh SR, et al. Amyrin attenuates scopolamine-induced cognitive impairment in mice. Biol Pharm Bull. 2014;37(7):1207-13.
β-Amyrin是一种天然存在的五环三萜类化合物，具有抗炎、抗菌、抗氧化作用。β-Amyrin能够有效阻断β-淀粉样蛋白诱导的长时程增强损伤[1-2]。β-Amyrin可用于阿尔茨海默病、癌症、肥胖症、动脉粥样硬化和II型糖尿病的相关研究[3-4]。
在体外，β-Amyrin（0-100μM）处理Hep-G2肝癌细胞24小时，可显著诱导细胞凋亡并引起G2/M期细胞周期阻滞，同时激活JNK和p38信号通路[5]。β-Amyrin（5μM）处理大肠杆菌（E. coli）细胞2小时。β-Amyrin通过诱导活性氧的积累和谷胱甘肽功能失调，引发细胞膜去极化，导致DNA断裂和磷脂酰丝氨酸外露诱导细胞发生凋死亡[6]。
在体内，β-Amyrin（4mg/kg）从Aβ（0.5mg；35-40μl；或1mg；70-80μl）注射后第1天至第5天每天口服给药，处理Aβ注射的阿尔茨海默病（AD）小鼠模型。β-Amyrin显著改善了由Aβ诱导的物体识别记忆和被动回避记忆损伤[7]。β-Amyrin（4mg/kg）在训练前60分钟口服处理被东莨菪碱诱导记忆损伤的ICR小鼠。β-Amyrin显著改善了由东莨菪碱引起的被动回避任务中的记忆损伤[8]。
| Cell experiment [1]: | |
Cell lines | Hep-G2 cells (human liver carcinoma cell line) |
Preparation Method | Hep-G2 cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO₂. Hep-G2 cells were treated with β-Amyrin (0-100μM) for 24 hours. |
Reaction Conditions | 0-100μM; 24h. |
Applications | β-Amyrin significantly induced apoptosis in Hep-G2 cells. β-Amyrin also caused cell cycle arrest at the G2/M phase. β-Amyrin activated the JNK and p38 signaling pathways. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | Mice were orally administered a single dose of β-Amyrin (4mg/kg) 60 minutes before the acquisition trial of a step-through passive avoidance task. Memory impairment was induced by an intraperitoneal (i.p.) injection of scopolamine (1mg/kg) 30 minutes before the acquisition trial. A retention trial was conducted 24 hours later. |
Dosage form | 4mg/kg; p.o.; single administration. |
Applications | A single administration of β-Amyrin (4mg/kg) significantly ameliorated the scopolamine-induced deficits in contextual long-term memory in the passive avoidance task. It also enhanced the phosphorylation levels of extracellular signal-regulated kinase (ERK) and glycogen synthase kinase-3β (GSK-3β) in the hippocampal tissue. |
References: | |
| Cas No. | 559-70-6 | SDF | |
| 别名 | β-香树脂醇,(-)-δ-Amyrin; δ-Amyrenol | ||
| Canonical SMILES | CC1(C)[C@]2([H])CC[C@@]3(C)[C@]4(C)CC[C@@]5(C)CCC(C)(C)C[C@@]5([H])C4=CC[C@]3([H])[C@@]2(C)CC[C@@H]1O | ||
| 分子式 | C30H50O | 分子量 | 426.7 |
| 溶解度 | DMF: 3 mg/ ml,DMF:PBS (pH 7.2) (1:8): 0.11 mg/ml,Ethanol: 1 mg/ml,Soluble in Chloroform | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3436 mL | 11.7178 mL | 23.4357 mL |
| 5 mM | 468.7 μL | 2.3436 mL | 4.6871 mL |
| 10 mM | 234.4 μL | 1.1718 mL | 2.3436 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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- Purity: >95.00% Appearance: A solid
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