Patchouli alcohol是一种天然的三环二半萜，来源于Pogostemon cablin Banth，具有抗菌、抗炎、抗肿瘤和抗病毒等多种生物学活性。
Cas No.:5986-55-0
Sample solution is provided at 25 µL, 10mM.
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Patchouli alcohol is a natural tricyclic diterpenoid derived from Pogostemon cablin Banth, possessing a variety of biological activities including antibacterial, anti-inflammatory, antitumor, and antiviral activity[1, 2]. Patchouli alcohol can inhibit viral replication during the early life cycle of influenza A virus infection and specifically prevent the expression of viral proteins hemagglutinin (HA) and neuraminidase (NA)[3].
In vitro, treatment of gastric cancer cell lines (NCI-N87 and HGC-27 cells) with Patchouli alcohol (0, 0.5, 1, 2µM) for 24-72h inhibited cell proliferation in a dose- and time-dependent manner, induced apoptosis and cell cycle arrest, and inhibited cell invasion and migration[4]. Treatment of DU145 and PC-3 cells with Patchouli alcohol (50-100µg/mL) for 24-72h inhibited cell proliferation, migration, and invasion, significantly downregulated the phosphorylation of NF-κB α and p65 inhibitors, and the expression of matrix metalloproteinases (MMP)-2, MMP-7, MMP-9, and vascular endothelial growth factor (VEGF)[5].
In vivo, treatment of A549 cell xenograft mice with Patchouli alcohol (5, 10, 15mg/kg) via intraperitoneal injection every 3 days for 21 days significantly inhibited tumor growth, significantly reduced the level of Ki67-positive cells in tumor tissue, and increased the level of cleavage caspase 3[6]. Oral administration of Patchouli alcohol (25, 50mg/kg) three times a week to obese mice induced by a high-fat diet significantly reduced body weight and significantly reduced fat accumulation in the epididymis and retroperitoneum[7].
References:
[1] Kumar A, Sharma N, Chanotiya C S, et al. The pharmacological potential and the agricultural significance of the aromatic crop Patchouli (Pogostemon cablin Benth.): A review[J]. Ecological Frontiers, 2024, 44(6): 1109-1118.
[2] Pandey S K, Gogoi R, Bhandari S, et al. A comparative study on chemical composition, pharmacological potential and toxicity of Pogostemon cablin Linn.,(Patchouli) flower and leaf essential oil[J]. Journal of Essential Oil Bearing Plants, 2022, 25(1): 160-179.
[3] Fan Y, Zhang Q, Zhang W, et al. Inhibitory effects of Patchouli alcohol on the early lifecycle stages of influenza A virus[J]. Frontiers in Microbiology, 2023, 13: 938868.
[4] Song Y, Chang L, Wang X, et al. Regulatory mechanism and experimental verification of patchouli alcohol on gastric cancer cell based on network pharmacology[J]. Frontiers in Oncology, 2021, 11: 711984.
[5] Cai J, Zhao J, Gao P, et al. Patchouli alcohol suppresses castration-resistant prostate cancer progression by inhibiting NF-κB signal pathways[J]. Translational Andrology and Urology, 2022, 11(4): 528.
[6] Lu X G, Yang L, Lu C H, et al. Molecular role of EGFR‐MAPK pathway in patchouli alcohol‐induced apoptosis and cell cycle arrest on A549 cells in vitro and in vivo[J]. BioMed research international, 2016, 2016(1): 4567580.
[7] Lee J, Kong B, Lee S H. Patchouli alcohol, a compound from Pogostemon cablin, inhibits obesity[J]. Journal of medicinal food, 2020, 23(3): 326-334.
Patchouli alcohol是一种天然的三环二半萜，来源于Pogostemon cablin Banth，具有抗菌、抗炎、抗肿瘤和抗病毒等多种生物学活性[1, 2]。Patchouli alcohol在甲型流感病毒感染的早期生命周期阶段能够抑制病毒复制，并特异性地阻止病毒蛋白血球凝集素（HA）和神经氨酸酶（NA）的表达[3]。
在体外，Patchouli alcohol（0, 0.5, 1, 2µM）处理胃癌细胞系（NCI-N87、HGC-27细胞）24-72h，以剂量和时间依赖性方式抑制了细胞增殖，诱导了细胞凋亡和细胞周期停滞，抑制了细胞的入侵和迁移[4]。Patchouli alcohol（50-100µg/mL）处理DU145和PC-3细胞）24-72h，抑制了细胞的增殖、迁移和侵入，显著下调了NF-κB α和p65抑制剂的磷酸化，以及基质金属蛋白（MMP）-2、MMP-7、MMP-9和血管内皮生长因子（VEGF）的表达[5]。
在体内，Patchouli alcohol（5, 10, 15mg/kg）每隔3天通过腹腔注射给药治疗A549细胞异种移植小鼠21天，显著抑制了小鼠体内肿瘤的生长，显著降低了肿瘤组织中Ki67阳性细胞的水平，并提高了裂解型caspase3的水平[6]。Patchouli alcohol（25, 50mg/kg）每周3次通过口服喂食高脂饮食诱导的肥胖小鼠，显著减轻了小鼠的体重，显著减少了附睾和腹膜后的脂肪积累[7]。
| Cell experiment [1]: | |
Cell lines | NCI-N87、HGC-27 cells |
Preparation Method | NCI-N87 and HGC-27 cells were digested with trypsin to make single-cell suspension followed by their seeding in a 96-well plate at 5×104 cells/well. Cells were incubated with different concentrations of Patchouli alcohol (0, 0.5, 1, 2µM) in a constant temperature incubator at 37°C for 24h, 48h and 72h, respectively. Cell viability was determined using the MTT assay. |
Reaction Conditions | 0, 0.5, 1, 2µM; 24, 48, 72h |
Applications | Patchouli alcohol inhibited the proliferation of gastric cancer cells in a dose- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | BALB/C nude mice |
Preparation Method | Mice were injected with A549 cells intraperitoneally with 1×107 cells per mouse. There were 4 randomly divided groups (6 mice each group): control group (vehicle) and Patchouli alcohol (PA) treatment group with 5, 10, 15mg/kg PA. PA standard crystal was dissolved in saline containing 5% DMSO. When the volume of the tumor reached 100mm3, 200µL 5% DMSO/saline solutions including increasing dose of PA were injected intraperitoneally into the right flank of nude mice in PA treatment group. The disinfecting saline in same volumes containing 5% DMSO was administrated into the vehicle group. PA was injected into all PA treatment groups every 3 days for 21 days. After the last treatment, the nude mice were put to death in 24h. Tumor xenografts removed from mice were measured and fixed for next experiment. Tumor sizes were evaluated every 3 days using micrometer calipers, and tumor weights were determined at last. |
Dosage form | 5, 10, 15mg/kg; 21 days; i.p. |
Applications | The tumor volume in the Patchouli alcohol treatment group was smaller than that in the control group. Patchouli alcohol treatment significantly reduced the level of Ki67-positive cells in the tumor tissue and increased the level of cleaved caspase 3. |
References: | |
| Cas No. | 5986-55-0 | SDF | |
| 别名 | 百秋李醇 | ||
| Canonical SMILES | O[C@@]1(C2(C)C)CC[C@H](C)[C@]3([H])C[C@@]2([H])CC[C@]13C | ||
| 分子式 | C15H26O | 分子量 | 222.37 |
| 溶解度 | DMSO: 125 mg/mL (562.13 mM) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.497 mL | 22.485 mL | 44.9701 mL |
| 5 mM | 899.4 μL | 4.497 mL | 8.994 mL |
| 10 mM | 449.7 μL | 2.2485 mL | 4.497 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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