Rebaudioside M is a natural non-caloric sweetener and an agonist of the class 1 sweet taste receptor (TAS1R) heterodimer formed by TAS1R2 and TAS1R3.1 It is one of the minor steviol glycosides isolated from S. rebaudiana leaves.2 Rebaudioside M increases intracellular calcium levels in HEK293 cells expressing human TAS1R2 and TAS1R3 (EC50 = 29.54 ?M) and has a relative sweetness potency 200-350 times that of sucrose.1,2 Rebaudioside M is metabolized by gut microbiota to steviol, a compound whose safety is widely studied.2,3,4
1.Choi, Y., Manthey, J.A., Park, T.H., et al.Correlation between in vitro binding activity of sweeteners to cloned human sweet taste receptor and sensory evaluationFood Sci. Biotechnol.30(5)675-682(2021) 2.Prakash, I., Markosyan, A., and Bunders, C.Development of next generation Stevia sweetener: Rebaudioside MFoods3(1)162-175(2014) 3.Purkayastha, S., Markosyan, A., Prakash, I., et al.Steviol glycosides in purified stevia leaf extract sharing the same metabolic fateRegul. Toxicol. Pharmacol.77125-133(2016) 4.Roberts, A., and Renwick, A.G.Comparative toxicokinetics and metabolism of rebaudioside A, stevioside, and steviol in ratsFood Chem. Toxicol.46(Suppl 7)S31-S39(2008)