Iristectorin B是从Iris tectorum中提取的一种天然异黄酮，具有抗癌作用。
Cas No.:94396-09-5
Sample solution is provided at 25 µL, 10mM.
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Iristectorin B is a natural isoflavone from Iris tectorum with anticancer effects [1]. Iristectorin B can exert anti-inflammatory activity by inhibiting the production of NO induced by lipopolysaccharide (LPS)[2]. Iristectorin B is widely used as an internal reference to develop ionic liquid ultrasonic-assisted extraction methods for the extraction and separation of related compounds[3].
In vitro, Iristectorin B (25µM) treatment for 24 hours significantly enhanced the survival of PC12 cells after glucose oxygen deprivation/reoxygenation (OGD/R) treatment, reduced cell apoptosis, and significantly decreased the expressions of TFR1, HMOX1, and SLC3A2[4].
References:
[1] Ruan L J, Yan B X, Wu Y Q, et al. Integrated Chemometric and Spectrum-effect analysis discriminates Belamcanda chinensis and Iris tectorum in treating A549 lung Cancer cells[J]. Journal of Chromatography B, 2025: 124875.
[2] Gao B, Ma Y, Zhang L, et al. Identification and characterization of the chemical components of Iris tectorum Maxim. and evaluation of their nitric oxide inhibitory activity[J]. Rapid communications in mass spectrometry, 2021, 35(1): e8959.
[3] Sun Y, Li W, Wang J. Ionic liquid based ultrasonic assisted extraction of isoflavones from Iris tectorum Maxim and subsequently separation and purification by high-speed counter-current chromatography[J]. Journal of Chromatography B, 2011, 879(13-14): 975-980.
[4] Zheng M, Zhou M, Lu T, et al. TMT and PRM Based Quantitative Proteomics to Explore the Protective Role and Mechanism of Iristectorin B in Stroke[J]. International Journal of Molecular Sciences, 2023, 24(20): 15195.
Iristectorin B是从Iris tectorum中提取的一种天然异黄酮，具有抗癌作用[1]。Iristectorin B可通过抑制脂多糖（LPS）诱导的一氧化氮产生来发挥抗炎活性[2]。Iristectorin B被广泛用作内参，以开发离子液体超声辅助提取方法，用于相关化合物的提取和分离[3]。
在体外，使用25µM的Iristectorin B处理PC12细胞24小时，显著增强了细胞在糖氧剥夺/复氧（OGD/R）处理后的存活率，减少了细胞凋亡，并显著降低了TFR1、HMOX1和SLC3A2的表达[4]。
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | PC12 cells were cultured in high-glucose DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin at 37℃ in the presence of 5% CO2. 100μl of sugar-free EBSS solution containing 10mM Na2S2O4 was added and incubated for 2h. The cells were replaced with normal medium for reoxygenation and continued to be cultured for 24h, constituting an induced OGD/R injury model to simulate ischemic brain injury. OGD/R was supplemented with normal culture medium containing Iristectorin B 6.25µM, 12.5µM, and 25µM for 24h. The, cell viability was analyzed. |
Reaction Conditions | 6.25µM, 12.5µM, and 25µM; 24h |
Applications | Iristectorin B treatment enhanced the cell viability of PC12 cells after OGD/R injury. |
| Cas No. | 94396-09-5 | SDF | |
| 别名 | 鸢尾甲苷 B；鸢尾新苷；鸢尾甲黄素A-7-O-葡萄糖苷 | ||
| Canonical SMILES | O=C1C(C2=CC=C(O)C(OC)=C2)=COC3=CC(O[C@@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=C(OC)C(O)=C13 | ||
| 分子式 | C23H24O12 | 分子量 | 492.43 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0307 mL | 10.1537 mL | 20.3075 mL |
| 5 mM | 406.1 μL | 2.0307 mL | 4.0615 mL |
| 10 mM | 203.1 μL | 1.0154 mL | 2.0307 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
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2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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