Hydroxocobalamin monohydrochloride

Animal experiment:

Rats: Rats are pretreated with sterile PBS (0.1 mL/kg i.v.) 30 min before administration of LPS (0.8 mg/kg i.v.). Fifteen minutes after administration of LPS, when the 125-mediated decrease in blood pressure is maximum, the rats are administered either PBS (0.1 mI/kg i.v.) or hydroxocobalamin (20-30 mg/kg i.v.). Blood pressure, heart rate and RNI are measured[1]. Mice: Adult male CD1 mice, 25-30 g body weight are allowed to acclimatize for at least 7 days prior to experiment on standard chip bedding. All animals are fed ad libitum and are not fasted before experiments. Mice are treated with 0.1 mL/25 g volume per weight ratio of single injection. The survival of animals is recorded 24 h after the treatment[4].

References:

[1]. Cheungpasitporn W, et al. High-dose hydroxocobalamin for vasoplegic syndrome causing false blood leak alarm. Clin Kidney J. 2017 Jun;10(3):357-362.
[2]. Wang H, et al. Oral vitamin B12 versus intramuscular vitamin B12 for vitamin B12 deficiency. Cochrane Database Syst Rev. 2018 Mar 15;3:CD004655.
[3]. Greenberg SS, et al. Hydroxocobalamin (vitamin B12a) prevents and reverses endotoxin-induced hypotension and mortality in rodents: role of nitric oxide. J Pharmacol Exp Ther. 1995 Apr;273(1):257-65.
[4]. Truong DH, et al. Prevention of hydrogen sulfide (H2S)-induced mouse lethality and cytotoxicity by hydroxocobalamin (vitamin B(12a)). Toxicology. 2007 Dec 5;242(1-3):16-22.