Hexa-His is an amino acid sequence tag composed of six consecutive histidine residues. Hexa-His achieves efficient protein purification based on immobilized metal ion affinity chromatography (IMAC) through the specific binding of the imidazole groups on histidine to metal ions. Hexa-His can be used in the purification of recombinant proteins, the study of protein-protein interactions, and research on protein-based supramolecular biopolymers[1-4].
References:
[1] Guo M, Lv M, Shao Y, et al. Bax functions as coelomocyte apoptosis regulator in the sea cucumber Apostichopus japonicus. Dev Comp Immunol. 2020 Jan;102:103490.
[2] Guo M, Chen K, Lv Z, et al. Bcl-2 mediates coelomocytes apoptosis by suppressing cytochrome c release in Vibrio splendidus challenged Apostichopus japonicus. Dev Comp Immunol. 2020 Feb;103:103533.
[3] Kummarapurugu AB, Hawkridge AM, Ma J, et al. Neutrophil elastase decreases SARS-CoV-2 spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomain from the epithelial surface. J Biol Chem. 2023 Jun;299(6):104820.
[4] Slagle AK, Ghiringhelli Borsa N, Wang K, et al. Factor H-related 1 and heparan sulfate architecture contribute to complement dysregulation in C3 glomerulopathy. Front Immunol. 2025 May 16;16:1589674.
Hexa-His是一种由连续六个组氨酸残基组成的氨基酸序列标签，Hexa-His通过组氨酸上的咪唑基团与金属离子特异性结合，实现基于固定化金属离子亲和层析的高效蛋白质纯化。Hexa-His可用于重组蛋白纯化、蛋白质相互作用研究以及蛋白质超分子生物聚合的相关研究[1-4]。