CY7 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 745/800nm, respectively[1]. The basic structure of CY7 is a cyclic compound with a conjugated double bond system. It has excellent fluorescence properties in the far-red region and good photostability[2]. CY7 is a cyanine compound with the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility, and relatively simple synthesis[3]. CY7 usually exists in the form of its active ester or azide, which enables it to be labeled with biological molecules such as proteins and nucleic acids through covalent bonds[4]. The optimal molar ratio of CY dye to protein is about 10:1[5]. CY7 has a wide range of applications. It is not only suitable for biomarkers and cell imaging, but also for nucleic acid detection, protein analysis, and drug screening[6]. The molecular weight of this product is 682.85. It is for research purposes only and is not suitable for food, drugs, medical devices, or cosmetics.
References:
[1] Zhang R, Yang J, Radford D C, et al. FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate[J]. Macromolecular bioscience, 2017, 17(1): 1600125.
[2] Yuan L, Lin W, Zheng K, et al. Far-red to near infrared analyte-responsive fluorescent probes based on organic fluorophore platforms for fluorescence imaging[J]. Chemical Society Reviews, 2013, 42(2): 622-661.
[3] Pandey R K, James N, Chen Y, et al. Cyanine dye-based compounds for tumor imaging with and without photodynamic therapy[J]. Heterocyclic Polymethine Dyes: Synthesis, Properties and Applications, 2008: 41-74.
[4] Gerowska M, Hall L, Richardson J, et al. Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis[J]. Tetrahedron, 2012, 68(3): 857-864.
[5] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes[J]. Nucleic Acids Research, 1994, 22(15): 3226-3232.
[6] Ntziachristos V, Schellenberger E A, Ripoll J, et al. Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V–CY7. 5 conjugate[J]. Proceedings of the National Academy of Sciences, 2004, 101(33): 12294-12299.
CY7是一种含花青（Cyanine）的荧光染料，常用于蛋白、抗体以及小分子化合物的标记，最大激发/发射波长分别为745/800nm[1]。CY7的基本结构是一个带有共轭双键体系的环状化合物，在远红区具有优异的荧光特性，具有较好的光稳定性[2]。CY7是一种菁类化合物，具有波长长、吸收和发射可调、消光系数高、水溶性好、合成相对简单等特点[3]。CY7通常以其活性酯或叠氮化物的形式存在，这使得它能够通过共价键与蛋白质、核酸等生物分子进行标记[4]。CY染料与蛋白的最佳摩尔比为10:1左右[5]。CY7的应用范围非常广泛，不仅适用于生物标记和细胞成像，还可用于核酸检测、蛋白质分析以及药物筛选等领域[6]。本产品分子量为682.85，仅供研究用途，不适用于食品、药品、医疗器械或化妆品。