Z-Gly-Gly-Leu-AMC is the substrate of the ClpP1 and ClpP2 enzymes of Mycobacterium tuberculosis, and is used to detect the enzyme activity when the activating peptide Z-Leu-Leu is present [1]. Z-Gly-Gly-Leu-AMC is a substrate of the proteasome and is commonly used to determine the multicatalytic protease complex activity in various components of Trypanosoma brucei brucei [2]. Z-Gly-Gly-Leu-AMC is often used as a hydrolysis substrate to screen for activation peptides of protease HslV, in order to study the activation mechanism of the proteasome[3].
References:
[1] Akopian T, Kandror O, Raju R M, et al. The active ClpP protease from M. tuberculosis is a complex composed of a heptameric ClpP1 and a ClpP2 ring[J]. The EMBO journal, 2012, 31(6): 1529-1541.
[2] Lomo P O, Coetzer T H, Lonsdale-Eccles J D. Localisation of a multicatalytic protease complex in Trypanosoma brucei brucei[J]. Journal of Tropical Microbiology and Biotechnology, 2010, 6(1).
[3] Ramachandran R, Hartmann C, Song H K, et al. Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY)[J]. Proceedings of the National Academy of Sciences, 2002, 99(11): 7396-7401.
Z-Gly-Gly-Leu-AMC是结核分枝杆菌ClpP1和ClpP2酶的底物，用于在激活肽Z-Leu-Leu存在时检测酶活性[1]。Z-Gly-Gly-Leu-AMC是蛋白酶体的底物，常用于测定Trypanosoma brucei brucei各组分中多催化蛋白酶复合物的活性[2]。Z-Gly-Gly-Leu-AMC常作为水解底物，用于筛选蛋白酶HslV的激活肽，以研究蛋白酶体的激活机制[3]。